铁过载对大鼠骨髓及肝脾组织的影响

Effect of Iron Overload on Bone Marrow, Liver and Spleen in Rats

目的:通过腹腔注射右旋糖酐铁建立铁过载大鼠模型,观察过量补铁对大鼠骨髓及肝脾组织的影响。方法:雄性Wistar大鼠40只随机分为:正常对照组、低剂量铁组、中剂量铁组和高剂量铁组。经隔日腹腔注射每次分别给予右旋糖酐铁0.9 mg、0.3 mg、9mg、18 mg,共干预6周。观察各组大鼠的生长发育状况并检测相关指标。结果:四组大鼠白细胞计数、红细胞计数、血红蛋白浓度以及血小板计数差异均无统计学意义(P>0.05)。骨髓外铁含量分析,中、高剂量铁组大鼠骨髓基质中均出现不同程度的铁蓄积,骨髓细胞外铁含量均显著高于正常对照组(P<0.05)。与正常对照组比较,中、高剂量铁组大鼠肝脏系数分别升高52%和148%(P<0.05),脾脏系数分别升高56%和100%(P<0.05)。与正常对照组比较,中、高剂量铁组大鼠肝组织铁分别升高154%和303%(P<0.05),脾组织铁分别升高40%和127%(P<0.05),血清铁含量分别升高35%和165%(P<0.05)。结论:过量补铁(腹腔给药)可使大鼠骨髓基质出现铁沉积,肝脏和脾脏脏器系数及其组织铁含量显著增加,导致铁在机体内过量蓄积。因此临床铁补充应防止过量长期用药。

Objective： To explore the effects of the iron dextran on bone marrow, liver and spleen in iron overload rats. Methods： Forty Wistar male rats were randomly divided into four groups including control group （0.9 mg）, low-dose iron group （0.3 mg）, middle-dose iron group （9 mg） and high-dose iron group （18 mg）, then were injected with intraperitoneal iron dextran every other day for six weeks. The general situation and the related indicators were detected. Results： There was no significant difference in white blood cells, red blood cells, hemoglobin and platelets among these groups （P 〉0.05）. Compared with the control group, the iron content of bone marrow in the middle-dose iron group and the high-dose iron group were significantly higher than that of the control group （P 〈 0.05）. Compared with the control group, the liver coefficient and the spleen coefficient of the middle-dose iron group and high-dose iron group was increased by 52%, 148%, 56% and 100% respectively （all P〈0.05）. Compared with control group, the iron concentration of the liver and spleen in the middle-dose iron group and high-dose iron group was increased by 52%, 148%, 40% and 127% （all P〈0.05）, and the serum iron was increased by 35% and 165% （P〈0.05）. Conclusions： Even the iron overload doesn＇t impact on peripheral blood of rats, but inhibits the function of rat bone marrow, liver and spleen significantly.