SHIP在炎症性肠病结肠组织中的表达及其临床意义

Expression and Its Significance of SHIP in Intestinal Mucosa of Inflammatory Bowel Disease

目的:观察SHIP在溃疡性结肠炎(UC)和克罗恩病(CD)患者结肠组织标本中的表达情况,探讨其在炎症性肠病发生过程中所起的作用及意义。方法:收集活动期UC患者,活动期CD患者,及结直肠癌旁正常粘膜组织(NC组)标本各20例。将活检标本进行苏木精-伊红染色及SHIP免疫组化染色观察;利用Western blot半定量比较分析SHIP蛋白表达及组间差异;Real-time RT-PCR分析SHIP在RNA水平的表达情况和组间差异。统计学处理采用Student's t检验。结果:免疫组化染色示UC组SHIP阳性表达积分为(7.20±2.53),CD组积分为(6.50±2.76),对照组积分为(1.10±0.74)。t检验组间比较UC组和CD组无统计学差异(t=0.59,P>0.05);而UC组与NC组(t=7.32,P<0.05),CD组与NC组(t=5.98,P<0.05),差异均有统计学意义。Western blot检测结肠组织SHIP表达,UC组SHIP相对表达量为(0.314±0.021),CD组(0.301±0.019),NC组(0.163±0.027)。UC和CD组表达无差异(t=1.44,P>0.05),而UC组,CD组与NC组相比表达明显升高(t=13.88、13.16,P均<0.05)。Real-time RT PCR检测UC组结肠粘膜SHIP m RNA相对表达量为(0.649±0.028),CD组为(0.645±0.021),NC组为(0.140±0.015)。同样,UC组与CD组没有统计学差异,而其相较对照组表达均升高(P<0.05)。结论:炎症性肠病患者结肠组织SHIP表达明显高于正常结肠组织,但其在溃疡性结肠炎和克罗恩病间没有明显差异;提示SHIP可能在炎症性肠病的发病中发挥重要作用。

Objective： To investigate the expresssion of SHIP in the intestinal mucosa of patients with ulcerative colitis （UC） or Crohn＇s disease（CD）, and explore its rote and relevance in the pathogenesis of inflammatory bowel disease（IBD）. Methods： The intestinal or colonic mucosa biopsy specimens were collected from 20 active UC patients and 20 active CD patients had enteroscopy examination, and 20 patients underwent colorectal surgery. The colon tissues were taken for hematoxylin-eosin staining and immunohistochemieal staining （SHIP） observation. Western blot was performed for detection of protein expression of SHIP in each group and qRT-PCR was used to detect SHIP expression at RNA level. The data were analyzed by Student＇s t test. Results： The integral of SHIP positive expres- sion by immunohistochemical staining was （7.20± 2.53） in UC group, （6.50± 2.76） in CD group, and （1.10± 0.74） in negative control group. There was no statistical significance between UC and CD groups （t=-0.59,P〉0.05）, but the differences between UC and NC（t=7. 32, P〈0.05 ）, CD and NC （t=5.98, P〈0.05 ） were statistically significant. The expression of SHIP in intestinal mueosa detected by west- em blot was （0.314± 0.021 ）, （0.301± 0.019）, （0.163± 0.027）respectively in UC, CD, NC groups. No difference was found between UC and CD（t=-1.44, P〉0.05）, but they were significantly higher than negative control（t=13.88, 13.16, P〈0.05）. The expression of SHIP mRNA analyzed by Real-time RT-PCR of three groups was （0.649± 0.028）, （0.645± 0.021 ）, （0.140± 0.015）in UC, CD, NC groups, respectively and the results of statistical analysis was the same as above. Conclusions： The expression of SHIP in intestinal mucosa of IBD patients was significantly higher than normal colonic tissue, but there was no difference between UC and CD patients; and it was supposed that SHIP was closely correlated with the pathogenesis of inflammatory bowel disease.