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Shikonin Promotes Skin Cell Proliferation and Inhibits Nuclear Factor-κB Translocation via Proteasome Inhibition In Vitro 被引量:6

Shikonin Promotes Skin Cell Proliferation and Inhibits Nuclear Factor-κB Translocation via Proteasome Inhibition In Vitro
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摘要 Background:Shikonin is a major active chemical component extracted from Lithospermi Radix,an effective traditional herb in various types of wound healing.Shikonin can accelerate granulomatous tissue formation by the rat cotton pellet method and induce neovascularization in granulomatous tissue.The purpose of the study was to investigate its mechanism of action in human skin cells.Methods:MTS assay was used to measure cell growth.The collagen type Ⅰ (COL1) mRNA expression and procollagen type Ⅰ C-peptide (PIP) production were detected by real-time quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay,respectively.Immunofluorescence and western blot analyses were carried out to investigate nuclear factor-κB (NF-κB) signaling pathway.Cell-based proteasome activity assay was used to determine proteasome activity.Results:In this study,we found that 10 μmol/L shikonin stimulated the growth of normal human keratinocytes and 1 μmol/L shikonin promoted growth of human dermal fibroblasts.However,shikonin did not directly induce COLI mRNA expression and PIP production in dermal fibroblasts in vitro.In addition,1 μmol/L shikonin inhibited translocation of NF-κB p65 from cytoplasm to nucleus induced by tumor necrosis factor-α stimulation in dermal fibroblasts.Furthermore,shikonin inhibited chymotrypsin-like activity of proteasome and was associated with accumulation ofphosphorylated inhibitor κB-α in dermal fibroblasts.Conclusions:These results suggested that shikonin may promote wound healing via its cell growth promoting activity and suppress skin inflammation via inhibitory activity on proteasome.Thus,shikonin may be a potential therapeutic reagent both in wound healing and inflammatory skin diseases. Background:Shikonin is a major active chemical component extracted from Lithospermi Radix,an effective traditional herb in various types of wound healing.Shikonin can accelerate granulomatous tissue formation by the rat cotton pellet method and induce neovascularization in granulomatous tissue.The purpose of the study was to investigate its mechanism of action in human skin cells.Methods:MTS assay was used to measure cell growth.The collagen type Ⅰ (COL1) mRNA expression and procollagen type Ⅰ C-peptide (PIP) production were detected by real-time quantitative reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay,respectively.Immunofluorescence and western blot analyses were carried out to investigate nuclear factor-κB (NF-κB) signaling pathway.Cell-based proteasome activity assay was used to determine proteasome activity.Results:In this study,we found that 10 μmol/L shikonin stimulated the growth of normal human keratinocytes and 1 μmol/L shikonin promoted growth of human dermal fibroblasts.However,shikonin did not directly induce COLI mRNA expression and PIP production in dermal fibroblasts in vitro.In addition,1 μmol/L shikonin inhibited translocation of NF-κB p65 from cytoplasm to nucleus induced by tumor necrosis factor-α stimulation in dermal fibroblasts.Furthermore,shikonin inhibited chymotrypsin-like activity of proteasome and was associated with accumulation ofphosphorylated inhibitor κB-α in dermal fibroblasts.Conclusions:These results suggested that shikonin may promote wound healing via its cell growth promoting activity and suppress skin inflammation via inhibitory activity on proteasome.Thus,shikonin may be a potential therapeutic reagent both in wound healing and inflammatory skin diseases.
出处 《Chinese Medical Journal》 SCIE CAS CSCD 2015年第16期2228-2233,共6页 中华医学杂志(英文版)
关键词 Cell Growth Human Dermal Fibroblasts KERATINOCYTES PROTEASOME SHIKONIN Cell Growth Human Dermal Fibroblasts Keratinocytes Proteasome Shikonin
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  • 1Andújar I,Rios JL,Giner RM,Recio MC.Shikonin promotes intestinal wound healing in vitro via induction of TGF-β release in IEC-18 cells.Eur J Pharm Sci 2013;49:637-41.
  • 2Kaith BS,Kaith NS,ChauhanNS.Anti-inflammatory effect of Arnebia euchroma root extracts in rats.J Ethnopharmacol 1996;55:77-80.
  • 3Pietrosiuk A,Skopinska-R6zewska E,Furmanowa M,Wiedenfeld H,Sommer E,Sokolnicka I,et al.Immunomodulatory effect of shikonin derivatives isolated from Lithospermum canescens on cellular and humoral immunity in Balb/c mice.Pharmazie 2004;59:640-2.
  • 4Ozaki Y,Sakaguchi I,Tujimura M,Ikeda N,Nakayama M,Kato Y,et al.Study of the accelerating effect of shikonin and alkannin on the proliferation of granulation tissue in rats.Biol Pharm Bull 1998;21:366-70.
  • 5Sakaguchi I,Tsujimura M,Ikeda N,Minamino M,Kato Y,Watabe K,et al.Granulomatous tissue formation of shikon and shikonin by air pouch method.Biol Pharm Bull 2001;24:650-5.
  • 6Staniforth V,Wang SY,Shyur LF,Yang NS.Shikonins,phytocompounds from Lithospermum erythrorhizon,inhibit the transcriptional activation of human tumor necrosis factor alpha promoter in vivo.J Biol Chem 2004;279:5877-85.
  • 7Lu L,Qin A,Huang H,Zhou P,Zhang C,Liu N,et al.Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition.Eur J Pharmacol 2011;658:242-7.
  • 8Yang H,Zhou P,Huang H,Chen D,Ma N,Cui QC,et al.Shikonin exerts antitumor activity via proteasome inhibition and cell death induction in vitro and in vivo.Int J Cancer 2009;124:2450-9.
  • 9Yoo HG,Lee BH,Kim W,Lee JS,Kim GH,Chun OK,et al.Lithospermum erythrorhizon extract protects keratinocytes and fibroblasts against oxidative stress.J Med Food 2014;17:1189-96.
  • 10Ishida T,Sakaguchi I.Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.Biol Pharm Bull 2007;30:928-34.

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