组胺H4受体基因拷贝数与特应性皮炎的相关性研究

Study on the association between copy- number variations of the human histamine H4 receptor gene and atopic dermatitis

目的探讨组胺H4受体(HRH4)基因拷贝数与特应性皮炎(AD)发病的相关性。方法从872例特应性皮炎患者及967例健康对照者中提取DNA和RNA,分别检测HRH4的基因拷贝数,统计分析与AD的相关性。结果 AD患者组的HRH4基因拷贝数高于正常对照组(P<0.05),在AD的发病患者中HRH4基因拷贝数的增加显著高于正常对照组(P<0.01),而HRH4基因拷贝数减少则与AD的发病无统计学意义(P>0.05)。结论 HRH4基因拷贝数变化与AD的发病具有相关性。

Objective To investigate the association between copy- number variations（ CNVs） of the HRH4 gene and the risk of atopic dermatitis（ AD）. Methods Genomic DNA and RNA were collected from 872 patients with AD and 967 healthy controls,and the CNVs levels of HRH4 were examined. Results Statistical differences were observed in AD patients compared with the controls（ P〈0. 05）. Amplification of HRH4 copy numbers significantly increased the risk of AD（ P〈0. 01）,and the deletions of HRH4 copy numbers were not associated with disease risk for AD（ P〉0. 05）. Conclusions CNVs of the HRH4 gene are associated with AD.