弧菌基因组中整合性接合元件(ICEs)的预测分析

Predictive analysis of integrative and conjugative elements(ICEs)in completely sequenced Vibrio genomes

整合性接合元件(ICEs)是一种广泛存在于细菌中可以自主移动的遗传元件。ICEs通过水平移动整合到弧菌的染色体上,所携带的新基因有助于宿主在特定生存环境下获得生长优势。为考察ICEs在弧菌生长中发挥的作用,采用Prf C位点与核心基因相关的特征蛋白,进行了26株弧菌SXT/R391ICEs的预测。结果表明,在全测序的26株弧菌中,只有3株霍乱弧菌的较大染色体上存在SXT/R391ICEs,对这3株霍乱弧菌上SXT/R391ICEs的抗性基因和热点区域进行比较分析,结果表明,3株霍乱弧菌的SXT/R391ICE的抗性基因和热点区域虽然都拥有类似的骨架结构,但从获取基因的得失情况可以判断,这3株菌SXT/R391ICE的进化优先关系。

Integrative and conjugative elements （ ICEs ） are selftransmissible mobile genetic elements that mediate horizontal gene transfer in bacteria. ICEs and related elements can constitute a large proportion of bacterial chromosomes, and bestow a wide range of phenotypes upon their host with carried gene cassettes. To study the role of SXT/R391 ICEs in the growth of Vibrio, SXT/R391 ICEs were identified in the 26 completely sequenced Vibrio genome using PrfC and their characteristic modules. The results showed that only SXT/R391 ICEs were found from 3 V. cholerae, and we comparatively analyzed the resistance genes and hotspots among 3 SXT/R391 ICEs. The region in resistance genes and hotspots had similar structure in 3 SXT/R391 ICEs, it could indicate the precedence relation in 3 SXT/R391 ICEs based on the genes which lost or gained.