绵羊肺炎支原体感染羊细胞因子的动态变化

Dynamic change of cytokines in sheep infected with Mycoplasma ovipneumoniae

为检测绵羊在感染绵羊肺炎支原体（MO）前后细胞因子的含量变化情况，将6只巴什拜羊和6只盘羊杂交羊人工感染MO，在感染前后分别采集静脉血，分离血清，用ELISA方法检测IL-1β、TNF—α、IL-8、IL-10及IFN-γ含量。结果显示：两组羊在感染后IL-1β浓度均升高，第21天巴什拜羊的IL-1β含量降低，且显著低于杂交羊（P〈0．05）；感染后两组的TNF—α含量逐渐升高，第14～21天巴什拜羊TNF-α含量开始下降，且第14天显著低于杂交羊（P〈0．05），第21天极显著低于杂交羊（P〈0．01）；感染后第14和21天，巴什拜羊的IL-8含量开始下降，而杂交羊则继续升高，且杂交羊IL-8含量均极显著高于巴什拜羊（P〈0．01）；两组羊的IL-10含量在第5天达到最高，且杂交羊极显著高于巴什拜羊（P〈0．01），而第14和21天巴什拜羊显著（P〈0．05）和极显著（P〈0．01）高于杂交羊；感染后两组羊的IFN-1含量逐渐升高，第14～21天，巴什拜羊的IFN-γ含量开始下降，而杂交羊则继续升高，第14和21天巴什拜羊显著（P〈0．05）和极显著（P〈0．01）低于杂交羊。结果提示：绵羊感染MO前后细胞因子变化明显，巴什拜羊和杂交羊细胞因子变化也有差异，这对研究支原体肺炎发病机理及免疫治疗有指导意义。

The aim of this study was to detect the dynamic change of serum cytokines in sheep infected with Mycoplasma Ovipneumoniae （MO）. Six Bashibai sheep （BS） and 6 Argali hybrid sheep （AHS） were artificially infected with MO. The serum concentrations of IL-1β, TNF-α, IL-8, IL-10 and IFN-α,/at different time points were assayed by ELISA. The results showed that IL-113 levels in two groups were elevated after infection, and the level in AHS was higher than those in BS. On the 21st days post-infection （ DPI）, the level of IL-1β in BS began to decline, and significantly lower than that in AHS （P〈0. 05）. TNF-α levels in two groups were increased after infection, and it reached highest on 7 DPI in BS. From 14 to 21 DPI, TNF-α level in BS declined, contrast to that in AHS, and TNF-α in AHS was higher than that in BS （P〈0.05 ; P〈0. 01 ）. On the 14th to 21st DPI, the level of IL-8 in BS began to decline, while the AHS was the opposite. IL-8 in AHS was higher than that in BS （P〈0.01）. The IL-10 reached the highest level on 5DPI in two groups, and IL-10 in AHS was higher than that in BS （P〈0. 01 ）. From 14 to 21DPI, IL-10 in AHS was lower than that in BS （P〈0. 05 ; P〈0. 01 ）. The contents of IFN -γwere elevated after infection in two groups. From 14 to 21 DPI, IFN-γin AHS was higher than that in BS （P〈0.05 ; P〈0. 01 ）, and IFN-γlevel declined in BS while that in AHS was elevated. It suggests that serum cytokines presented obvious changes in sheep after infection, and there exists a significant difference between two different species of sheep. This study is of guiding significance in investigating MO pathogenesis and its immune therapy.