摘要
目的探讨小鼠感染疟疾后是否会对艾滋病疫苗的免疫产生影响。方法用HIV-1 DNA疫苗p VAX-gag分别免疫感染过疟疾和未感染过疟疾的Balb/c小鼠,观察不同组小鼠接种p VAX-gag疫苗后,细胞免疫、体液免疫和免疫记忆的变化。结果p VAX1-gag免疫感染过疟疾和未感染过疟疾的Balb/c小鼠,均可诱导出明显的体液免疫和细胞免疫反应;但p VAX1-gag免疫未感染过疟疾的小鼠诱导出的体液免疫反应高于感染过疟疾的小鼠,所获得的抗体滴度是感染过疟疾的小鼠的3倍,未感染过疟疾的小鼠诱导出的细胞免疫反应高于感染过疟疾的小鼠。结论感染疟疾后对艾滋病疫苗的免疫有一定的抑制作用,这为艾滋病疫苗的研发提供了实验依据。
DNA vaccines, exhibiting potential advantages over conventional vaccines for their simplicity and versatility, can induce specific humoral and cellular immune responses. Because of the overlap in many regions of the malaria and HIV-1 infection epidemic, there is a significant risk of co-infection in many areas, such as Guangxi and Yunnan Province of China. The present study designed to investigate the organism malaria infection on immune efficacy of AIDS vaccine. Malaria infected and uninfected Balb/c mice were immunized with the HIV-1 DNA vaccine pVAX-gag to observe the humoral immunity by ELISA, cellular immunity by ELISPOT and immune memory effect by cell proliferation assay kit. The results showed that HIV-1 DNA vaccine pVAX-gag could induce humoral and cellular immune responses both in malaria infected and uninfected groups of Balb/c mice. Furthermore, the humoral and the cellular immune response both much higher in uninfected mice than those in malaria infected mice, and immunization with the HIV-1 DNA vaccine pVAX-gag in uninfected Balb/c mice induced 3.0-fold greater gag-specific humoral responses than that in the malaria infected mice. Taken together, malaria infection has a certain inhibition effect to AIDS vaccine, and our research provides an experimental basis for the development of HIV-1 vaccines.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2015年第9期748-752,共5页
Immunological Journal
基金
重庆市自然科学基金(cstc2012jj A10108)
广州医科大学科研项目
