Sn在PRRSV免疫复合物感染PAM细胞中的作用

Effects of Sn antibody in immune complex PRRSV infection to PAMs

目的研究猪唾液酸黏附素(Sn)受体在抗体介导PRRSV感染PAM细胞中的作用。方法用已经纯化的猪抗PRRSV Ig G与TCID50104.6/0.1 ml的PRRSV Hn-3/06株等体积混合均匀制成感染性免疫复合物,用Sn Ig G和Sn Ig G+CD163 Ig G分别封闭PAM细胞,然后用感染性免疫复合物感染封闭后的PAM细胞,并设鼠阴性Ig G封闭组作对照,48 h后收集样品。用已建立的PRRSV SYBR GreenⅡ实时荧光定量PCR方法检测PRRSV的m RNA水平,并运用Graph Pad Prism 5软件处理数据。结果单独Sn封闭及联合封闭Sn和CD163后,PRRSV-Ig G组病毒含量不但没有升高,反而下降,且联合封闭下降更明显。结论 Sn受体可以在一定程度上调节感染性免疫复合物对机体的作用,对其进入宿主靶细胞具有协助和促进作用。

This study aimed to evaluate the role of Sn antibody in porcine reproductive and respiratory syndrome virus （PRRSV） infection to pulmonary alveolar macrophage cells （PAMs）. Purified specific PRRSV IgG was mixed with an equal volume of PRRSV Hn-3/06 virus with TCID50 10^4.6/0.1 ml to prepare infectious immune complexes. Mouse anti-pig IgG of Sn and Sn＋CD163 were used to block PAM cells, which then infected with the infectious immune complexes, with mouse IgG negative group as control. After culture respectively for 48 h, PAM cells and the supernatant were collected, and the viral mRNA levels of infected cells were detected with established PRRSV SYBR Green II real-time PCR method, and using the GraphPad Prism 5 software to handle with the data we got. Data showed that the virus content was declined after antibodies block, and this phenomenon was more obvious in Sn＋CD163 block group. In conclusion, Sn can regulate the effects of infectious immune complexes to bodies to some extent, and help immune complexes to enter into host target cells.