白藜芦醇促进肝部分切除术后肝再生的实验研究

Resveratrol promotes the liver regeneration after partial hepatectomy

目的研究白藜芦醇对小鼠70%肝切除后残余肝的再生是否有促进作用。方法实验动物为雄性C57BL/6小鼠。将100只小鼠随机分为实验组(白藜芦醇预处理组)和对照组(生理盐水预处理组)。采用肝大部分切除术建立肝再生模型,术前连续5 d分别给予小鼠腹腔内注射白藜芦醇12 g/kg(实验组)和生理盐水(对照组),第5天注射完白藜芦醇和生理盐水2 h后给两组小鼠分别进行70%的肝切除手术(pH)。用肝重/体重比,实时定量聚合酶链式反应及免疫组化等方法来评估白藜芦醇对小鼠肝再生的促进作用。结果 pH术后36 h、48 h实验组与对照组相比,肝重/体重比增高(4.56±0.07对3.93±0.07;5.36±0.07对4.6±0.09)。肝脏ki-67术后36 h表达最为活跃,48 h后下降,实验组与对照组相比ki-67表达明显增高。实验组中组织肝细胞生长因子(HGF)及肿瘤坏死因子(TNF-α)水平明显比对照组增强。结论白藜芦醇能明显促进小鼠部分肝切除后的肝再生。

Objective To explore whether resveratrol can promote liver regeneration after 70% partial hepatectomy and to provide an animal model for clinical investigation of drugs for liver regeneration .Methods One hundred C57BL/6 mice were randomly divided into 2 groups ：experimental group and control group .Mice in the control group were given 0 .9% saline by intraperitoneal injection ,while the mice in experimental group were given resveratrol for 5 days .Mice were all subjected to 70% pH 2 h after their last injection of 0 .9% saline or resveratrol .We assessed the liver generation by liver weight/body weight ratio ,real‐time PCR and immunochemical staining of Ki‐67 .Results After pH 36 h and 48 h ,the liver weight/body weight ratio of the experimental group increased compared with the control group （4 .56 ± 0 .07 vs 3 .93 ± 0 .07 ;5 .36 ± 0 .07 vs 4 .6 ± 0 .09） .Liver ki‐67 expression was most active after 36 h ,and then fall after 48 h ,the ki‐67 expression of the experimental group was significantly increased .The tissues hepatocyte growth factor （HGF） and tumor necrosis factor （TNF‐α） levels of experimental group are significantly higher than the control group . Conclusion Resveratrol could obviously promote liver regeneration after 70% liver hepatectomy .