人参皂甙单体Rb1对大鼠脑缺血损伤保护作用的实验研究

Experimental study on the protection of ginseng Rb1 in rats with cerebral ischemic reperfusion injury

目的观察人参皂甙单体Rb1对大鼠实验性脑缺血的保护作用。方法健康成年雄性清洁级SD大鼠随机分为假手术组(Sham)、缺血对照组(Con)、干预组(Tre),干预组又分为Rb130 mg/kg、60 mg/kg及90 mg/kg三个不同剂量组。缺血对照组采用线栓法建立大脑中动脉闭塞模型,缺血2 h后拨出线栓再灌注;各干预组用相应剂量Rb1ip qd×7 d,末次给药后3 h内用同样方法建立大脑中动脉闭塞模型及再灌注;假手术组不插入线栓,余操作相同。术后48 h取标本TTC染色测梗死体积、干湿重法脑组织含水量测定、Tunnel法测定调亡细胞数及NgR表达的免疫组化测定。结果各干预组的梗死体积分别为30 mg/kg组(27.629±1.401)%,60 mg/kg组(24.164±1.710)%,90mg/kg组(21.955±2.556)%,缺血对照组为(29.846±1.153)%;脑组织含水量为30 mg/kg组(80.079±0.726)%,60 mg/kg组(78.984±0.902)%,90 mg/kg组(77.855±0.258)%,假手术组与缺血对照组分别为(76.517±0.37)%、(81.799±1.065)%;调亡细胞数分别为30 mg/kg组(89.000±10.296),60 mg/kg组(59.000±12.522),90 mg/kg组(36.667±19.054),假手术组与缺血对照组分别为(1.600±1.517)、(132.667±22.223);NgR表达阳性面积分别为30 mg/kg组(84.827±3.870),90 mg/kg组(66.040±5.541),60 mg/kg组(75.577±7.150),假手术组与缺血对照组分别为(48.355±9.720)、(91.485±5.822)。结论人参皂甙单体Rb1对大鼠实验性脑缺血有保护作用,能减轻缺血再灌注损伤所致的脑水肿及梗塞面积,减少细胞调亡,并且该保护作用呈剂量依赖性。对NgR表达的干预提示Rb1可能在脑缺血死后神经可塑性中起促进作用。

Objective To observe the protection of ginseng Rbl in rats with local cerebral ischemic reperfusion injury. Methods Healthy Sprague-Dawley rats were divided into three groups： Sham, control and treat group. Treat group was divided into three subgroups ： Rb130 rng/kg, 60 mg/kg and 90 mg/kg group. MCAO models were created after seven days of Rbl ip qd in treat group with respective dose and NS ip qd in control group. Tthe line extracted after occlusion 2 h. Sham group only receives sham operation. Rats were sacrificed at 48 h. The markers such as the expression intensity of NgR protein, brain water content, volume of infarction by TTC staining, and the count of apoptosis cell were estimated. Results Compared with control groups, the levels of expression of NgR protein, brain water content, volume of infarction and the count of apoptosis cell were lower in treat group and showed statistical difference （P（0. 05）. In treat group, there was also statistical difference between three subgroups. Conclusions The article show ginseng Rbl has protective effect on rats with local cerebral ischemic reperfusion injury. Rbl can decrease brain edema, infarction volume and apoptosis cell counting induced by local ischemic perfusion injury. It also indicates Rbl may play a role in rehabilitation after CNS injury because Rbl reduced the expression of NgR protein.