Cav-1在胰腺癌中表达及与临床预后关系的研究

Loss of stromal caveolin-1 expression: a novel tumor microenvironment biomarker that can predict poor clinical outcomes for pancreatic cancer

目的:肿瘤的发生发展不仅与肿瘤细胞的增殖有关,还依赖于肿瘤细胞和间质微环境之间的相互作用,对肿瘤微环境起关键调节作用的间质小窝蛋白-1(Cav-1)可能是一个潜在的治疗靶点,本研究旨在探讨胰腺癌间质Cav-1的表达能否作为客观的预后标志物。方法:选取45例胰腺癌标本,应用免疫组化法测定胰腺癌、癌旁及正常组织间质Cav-1表达,分析间质Cav-1的表达与临床病理特征和预后的相关性。结果:6例(13.3%)标本间质Cav-1呈强染色,8例(17.8%)呈低-中度染色,31例(68.9%)无染色。间质Cav-1表达缺失与TNM分期(P=0.018)、淋巴结转移(P=0.014)、远处转移(P=0.027)相关,与年龄、性别、组织学分级和肿瘤大小无显著性相关(P>0.05)。结论:胰腺癌间质Cav-1是独立预后指标,可作为胰腺癌有效治疗的靶标。

Objective： Cancer development and progression is not only associated with the tumor cell proliferation but also depends on the interaction between tumor cells and the stromal microenvironment. A new understanding of the role of the tumor microenvironment suggests that the loss of stromal caveolin-1（ Cav-1） as a key regulator may become a potential therapy target. This study aims to elucidate whether stromal Cav-1 expression in pancreatic cancer can be a strong prognosis biomarker. Methods： Tissue samples from 45 pancreatic cancer patients were studied. Stromal Cav-1 expression was measured from pancreatic cancer,paraneoplastic,and normal tissue by using immunohistochemistry. We analyzed the correlation of stromal Cav-1 expression with clinicopathologic features and prognostic. Results： Specimens from six patients（ 13. 3%） showed high levels of stromal Cav-1 staining,those from eight patients（ 17. 8%） showed a lower,intermediate level of staining,whereas those from 31 patients（ 68. 9%） showed an absence of staining. Stromal Cav-1 loss was associated with TNM stage（ P = 0. 018）,lymph node metastasis（ P = 0. 014）,distant metastasis（ P = 0. 027）. The relationships of age,sex,histological grade,and tumor size with stromal Cav-1 expression were not significant（ P〉0. 05）. Conclusion： The loss of stromal Cav-1 in pancreatic cancer is an independent prognostic indicator,thus suggesting that stromal Cav-1 may be an effective therapeutic target for patients with pancreatic cancer.