钩藤总碱对高血压动脉压力感受反射和对高血压靶器官的作用

Effects of Total Alkaloids of Rhynchophylline on Baroreflex and Hypertensive Target Organs in Spontaneous Hypertensive Rats

目的观察钩藤总碱对高血压靶器官的作用。方法自发性高血压模型大鼠40只,随机分为4组,正常对照组、天麻钩藤饮组、钩藤总碱小剂量组和钩藤总碱大剂量组,每组10只,分别口服普通饲料、天麻钩藤饮颗粒(750 mg·d-1)、钩藤总碱2.5,15.0 mg·d-1。采用改良的Smyth方法测定各组大鼠的动脉压力感受反射功能,20周后,取大鼠心、脑、肾,进行组织学观察。结果正常对照组、天麻钩藤饮组、钩藤总碱小剂量组和钩藤总碱大剂量组第16周动脉压力感受反射敏感性分别为(0.27±0.05),(0.31±0.06),(0.35±0.08),(0.34±0.08)ms·mm Hg-1。正常对照组大鼠动脉压力感受反射功能降低,而其他各用药组没有降低;给药组大鼠可见心肌结构显著优于正常对照组,脑组织和肾小球结构未见显著改善。结论钩藤总碱对高血压靶器官心脏有保护作用。

Objective To investigate the effect of total alkaloids from Rhynchophylline on hypertensive target organs.Methods 40 spontaneous hypertensive rats( SHRs) were divided into 4 groups as follows: normal control group,positive control with Tianma Goutengyin granule at 750 mg·d-1,total alkaloids from Rhynchophylline at 2.5 mg·day-1as low dosage,and at 15.0mg·d-1as high dosage.All the rats were fed up for 20 weeks,and baroreflex sensitivity( BRS,in ms·mm Hg-1) was determined by revised Smyth's method. Heart,brain and kidney of the rats were sectioned for histological analysis at the end of 20 th week.Results In sixteenth weeks,BRS of normal control,Tianma Goutengyin granule group,total alkaloids at low dosage and high dosage were( 0.27±0.05),( 0.31±0.06),( 0.35±0.08),( 0.34±0.08) ms·mm Hg-1,respectively.BRS in SHR was not decreased except in the normal control group.The treatment groups were superior to the normal control group in structure of heart,but not in the brain and kidney of the rats. Conclusion Total alkaloids from Rhynchophyllinecould protect heart from hypertensive injury.