响应面法优化白芍提取工艺的研究

Optimization of extraction technology from Paeoniae Radix Alba using response surface methodology

为进一步优化白芍提取工艺,从化学成分稳定性和操作便捷性考虑,该实验选择超声法对白芍提取工艺进行研究。以液相色谱图中共有峰的总峰面积为指标,通过单因素法考察超声时间、乙醇浓度、液料比对白芍提取效果的影响,并运用响应面法优化白芍的超声提取工艺。实验结果表明,乙醇浓度对白芍提取效果的影响最大,其次是液料比与超声时间。响应面法优化得白芍超声提取工艺的最适条件为:超声时间20.06 min、乙醇浓度72.04%、液料比53.38 m L·g-1;色谱图中共有峰总峰面积的模型预测值为2.160 8×108。通过验证实验发现:在此最佳提取条件下,色谱图中共有峰总峰面积的实际值为2.142 2×108,与预测值相近,二者相对偏差为0.86%,说明响应面优化所得的白芍提取工艺稳定、可行。从白芍提取效果完全、充分的角度看,该研究所得工艺较《中国药典》所载"白芍"中芍药苷提取方法更具优势,可用于白芍的开发、利用和质量综合评价。

To ensure the stability of chemistry components and the convenience of operation,ultrasound method was chosen to study in this investigation. As the total common peaks area in chromatograms was set to be evaluation index,the influence on the technology caused by extraction time,ethanol concentration and liquid-to-solid ratio was studied by using single factor methodology,and the extraction technology of Paeoniae Radix Alba was optimized by using response surface methodology. The results showed that the extracting results were most affected by ethanol concentration; liquid-to-solid ratio came the second and extraction time thirdly. The optimum ultrasonic-assisted extraction conditions were as follow： the ultrasonic extraction time was 20. 06 min,the ethanol concentration in solvent was 72. 04%,and the liquid-to-solid ratio was 53. 38 m L·g-1,the predicted value of total common peaks area was 2. 160 8 × 108.Under the extraction conditions after optimization,the total common peaks area was 2. 142 2 × 108,and the relative deviation between the measured and predicted value was 0. 86%,so the optimized extraction technology for Paeoniae Radix Alba is suitable and feasible.Besides,for the purpose of extracting more sufficiently and completely,the optimized extraction technology had more advantages than the extraction method recorded in the monogragh of Paeoniae Radix Alba in Chinese Pharmacopoeia,which will come true the assessment and utilization comprehensively.