木蝴蝶种子的化学成分研究

Study on chemical constituents from seed of Oroxylum indicum

采用正反相硅胶,Sephadex LH-20,MCI树脂等常规柱色谱及高效液相色谱技术对中药木蝴蝶种子80%甲醇提取物进行化学成分的分离纯化,从中分离得到11个化合物,根据其理化性质以及核磁波谱数据鉴定化合物结构为木蝴蝶苷B(1)、白杨黄素(2)、黄苓苷元(3)、5,6-二羟基-7-甲氧基黄酮(4)、槲皮素-3-O-β-D-半乳糖苷(5)、槲皮素-7-O-β-D-葡萄糖苷(6)、2α,3β-二羟基羽扇豆醇(7)、羽扇豆醇(8)、顺式-连翘环己醇(9)、β-谷甾醇(10)、豆甾醇(11)。其中,化合物5为首次从木蝴蝶中分离得到。

Oroxylum indicum was a traditional Chinese medicine. In order to study the chemical constituents from the seed of O. indicum,the chemical constituents of 80% methanol extract of seeds of O. indicum were subjected to chromatography on silica gel,Sephadex LH-20,and preparative HPLC,leading to the isolation of eleven compounds. The structures were identified by various spectroscopic data including ESI-MS,1H-NMR and13C-NMR data as oroxin B（ 1）,chrysin（ 2）,baicalein（ 3）,neglectein（ 4）,quercetin-3-O-β-D-galactopy ranoside（ 5）,quercetin-7-O-β-D-glucopyranoside（ 6）,2α,3β-dihydroxyllu Peol（ 7）,lupeol（ 8）,rengyol（ 9）,β-sitostero（ 10）,and stigmasterol（ 11）. Among them,compound 5 were firstly obtained from O. indicum.