槲皮素-3-葡萄糖苷对肾缺血-再灌注损伤大鼠的保护作用

Protective effects of quercetin-3- grape glycosides on renal ischemia-reperfusion injury in rats

目的:观察槲皮素-3-葡萄糖苷对急性肾缺血-再灌注损伤大鼠的保护作用。方法:采用夹闭大鼠双侧肾动脉45min后再灌注60min的方法,建立大鼠急性肾缺血-再灌注损伤的动物模型。通过测定血清丙二醛(MDA)、超氧化物歧化酶(SOD)、血清肌酐(Cr)、尿素氮(BUN),和TUNEL法分析肾小管上皮细胞的凋亡情况,探讨槲皮素-3-葡萄糖苷对急性肾缺血-再灌注损伤大鼠的保护作用及可能机制。结果:槲皮素-3-葡萄糖苷组MDA值显著降低(P<0.05);血清SOD含量升高明显;血清NO含量较模型组明显下降,两组比较有显著性差异(P<0.05)。槲皮素-3-葡萄糖苷组的TUNEL阳性细胞数与模型组相比明显减少(P<0.05)。结论:槲皮素-3-葡萄糖苷可以减轻肾小管细胞的损伤,对肾缺血-再灌注损伤大鼠具有保护作用,其作用机制可能是通过清除氧自由基,抑制脂质过氧化,进而改善肾功能。

Objective： To observe protective effects of quercetin -3- grape glycosides on acute renal ischemia reperfusion injury in rats. Methods： A rat model of acute renal ischemia reperfusion injury was established by the 60min method after the 45min of the bilateral renal artery. Through the determination of serum malondialdehyde （MDA）, superoxide dismutase （SOD）, serum creatinine （CR）, blood urea nitrogen （BUN）, and TUNEL assay of apoptosis of renal tubular epithelial cells and the protection and possible mechanism of Quercitrin-3-glucoside on acute renal ischemia - reperfusion injury in rats were investigated. Results： In Quercitrin-3-glucoside group, MDA decreased significantly （P〈0.05）; serum SOD content increased significantly; compared with the model group, the content of serum NO decreased significantly, with significant difference （P〈0.05）. The number of TUNEL positive cells was significantly decreased （P〈0.05）. Conclusion： Quercitrin-3-glucoside can alleviate the injury of renal tubular cells, has a protective effect on renal ischemia - reperfusion injury in rats. The mechanism may improve renal function by scavenging oxygen free radicals and inhibiting lipid peroxidation.