小鼠对于高脂诱导的肥胖的易感性与肠道菌群的关系

Correlation between the susceptibility to high-fat diet-induced obesity and gut microbiota in mice

目的探究肠道菌群与小鼠在高脂膳食下形成的不同肥胖表型的关系。方法高脂饲料构建肥胖抵抗(DIO-R)和肥胖易感(DIO-P)模型,正常饲料组为对照(NC),使用末端限制性片段长度多态性(T-RFLP)和荧光实时定量PCR(RT-PCR)技术分析小鼠初始(0周)和干预后(7周)的肠道菌群。结果不仅在表型差异形成后,DIO-R和DIO-P两组小鼠的肠道菌群差异具有统计学意义,而且在初始时刻,不同肥胖表型小鼠的肠道菌群就存在不同:0周和7周时,DIO-P组和DIO-R组在酶切片段的主成分分析图上均可以明显区分,且两组小鼠肠道中Lactobacillus、Bifidobacterium、Akkermansia muciniphila、Desulfovibrio、Staphylococcus aureus、Enterobacter cloacae六种细菌的定量结果也具有明显的差别。结论肠道菌群初始状态的差异可能引导小鼠摄入高脂后对于肥胖的易感性。

Objective To explore the relationship between the susceptibility to high-fat diet-induced obesity and the composition of gut microbiota in mice.Methods High-fat diet was used to build diet-induced obesity-resistant（DIO-R）and diet-induced obesity-prone（DIO-P）models,with mice fed with Normal feed as the controls（NC）.Terminal restriction fragment length polymorphism（T-RFLP）and real-time quantitative PCR（RT-PCR）were used to analyze the gut microbiota before（at Week 0）and after（at Week 7）the intervention.Results Significant differences in gut microbiota between DIO-R and DIO-P mice were observed not only after the formation of different obese phenotype,but also from the very beginning：at Week 0or 7,the DIO-P and DIO-R were clearly distinguished on the analytical chart of principal components of T-RFs.The quantitative results of Lactobacillus,Bifidobacterium,Akkermansia muciniphila,Desulfovibrio,Staphylococcus aureus and Enterobacter cloacae of the two groups also had significant differences.Conclusion The initial gut microbiota may lead to the susceptibility to obesity in mice when they are fed high-fat diet.