江口萝卜猪FABPs主要家族基因SNPs筛选及生物信息学分析

SNP Screening and Bioinformatics Analysis of the Main Family Genes of FABPs in Jiangkou Luobo Pig

以江口萝卜猪为试验材料构建DNA池,采用直接测序技术对猪L-FABP、I-FABP、H-FABP、A-FABP基因进行SNPs快速筛查,共检测出7个SNPs,分别为intron1-T1745C、exon2-T125C、exon2-A131C、exon2-C153A、exon2-A65T、exon2-G40A、intron3-C65T。其中intron1-T1745C、intron3-C65T位于内含子上;exon2-T125C、exon2-C153A为同义突变;exon2-A131C、exon2-A65T、exon2-G40A为错义突变,分别使编码氨基酸发生Glu→Ala、Lys→Met、Glu→Lys的改变,对其进行生物信息学分析表明,突变前后的等位基因频率估算,mR NA二级结构预测,蛋白质二级、三级结构预测分析均有差异。

Jian gkou Luobo Pigs were used to construct DNA pool,the SNPs in genes of L-FABP,I-FABP,H-FABP and A-FABP were rapidly screened by DNA sequencing,seven SNPs were detected in the four genes which included two SNPs（intron1-T1745 C,intron3-C65T） located in the intron,two SNPs（exon2-T125 C,exon2-C153A） were synonymous mutation,and three SNPs were missense mutations（exon2-A131 C,Glu→Ala; exon2-A65 T,Lys→Met; exon2-G40 A,Glu→Lys）.The bioinformatics analysis indicted that the missense mutations could cause the change of allele frequency,mR NA secondary structure,secondary and tertiary protein structure.