藏药洮河红景天trnS-G基因与红景天苷含量变异的相关性研究

Correlation Analysis between Gene Polymorphism of trnS-G Sequence and Content of Salidrosides in Tibetan Medicine of Rhodiola taohoensis S.H.Fu

本研究采集不同地区的洮河红景天植株,采用改良CTAB法提取总DNA。采用PCR方法扩增trn S-G基因片段,对扩增产物进行序列测定,分析红景天trn S-G基因多态性。同时,采用高效液相色谱法（HPLC）测定不同洮河红景天红景天苷含量,分析红景天苷含量差异。结果表明：不同洮河红景天trnS-G基因序列存在多样性。扩增得到的目的序列长度在597~600 bp之间。8个洮河红景天样品中,trnS-G基因片段可分为5个单倍型,其中：H1、H2、H3单倍型对应的样品均未检测到红景天苷的存在,H4、H5单倍型样品中检测到红景天苷的积累,H4单倍型样品的平均含量为0.18%,H5号单倍型仅有1个样品,含量为0.2%。洮河红景天trnS-G基因多态性与地理来源有一定的相关性,trnS-G单倍型与红景天苷含量具有相关性。

Rhodiola taohoensis S.H.Fu,a Tibetan medicinal plant was collected from different areas in Qinghai Province.The total DNA was extracted by modified CTAB method.trn S-G gene fragments were amplified by PCR and were sequenced to analyze the trn S-G gene polymorphism.Salidroside content in different samples of R.taohoensis S.H.Fu was determined by HPLC.The Results showed that the size of target sequence amplified ranges from 597 to 600 bp in length.The trn S-G genes can be divided into five genotypes in the eight R.taohoensis samples,and salidroside was not detected in the samples of H1,H2 and H3 genotype.The average salidroside content of H4 genotype is 0.18%,and the salidroside content of the only sample of H5 genotype is 0.2%.We identified that there is some degree of correlation between trn S-G gene diversity and geographical origin,at the same time,correlation exists in the trn S-G gene diversity and the content of salidroside.