甘蔗KNOX基因(Sckn1)的电子克隆及生物信息学分析

In Silico Cloning and Bioinformatics Analysis of KNOX Gene in Sugarcane(Sckn1)

KNOX基因对植物顶端分生组织的形成和维持有至关重要的作用。本文选择功能已知的水稻KNOX基因(OSH1,Oskn1)为参考序列,采用电子克隆技术克隆到甘蔗中与OSH1同源的一个KNOX基因Sckn1,并通过生物信息学分析预测该基因的结构、功能。结果表明:该基因包含一个1 071 bp的开放阅读框,编码356个氨基酸,分子量为39.36 kD,理论等电点为6.47。该基因很可能定位于细胞核,起转录调控作用,序列比对发现该基因编码蛋白与高粱、玉米、小米草等KN1蛋白高度相似。以上分析结果为进一步研究Sckn1基因的功能奠定了坚实的基础。

KNOX plays an important role in the formation and maintenance of plant apical meristem.We chose rice KNOX gene（OSH1,Oskn1） as the reference sequence to clone the KNOX gene that is homologous with OSH1 from sugarcane（named Sckn1） based on in silico cloning approach,then,the structure and function of Sckn1 was analyzed and predicted by bioinformatics.The results showed that Sckn1 contains a 1 071 bp open reading frame encoding 356 amino acids with the molecular weight 39.36 kD and theoretical isoelectric point 6.47.This gene is likely to be located in the nucleus,playing a role of transcriptional regulation.Sequence alignment revealed that Sc KN1 and KN1 from sorghum,zea mays,setaria italica should be of great similar.These analyses lay a solid foundation for further research of Sckn1.