椒江口沉积物中细菌多样性初步研究

BACTERIAL DIVERSITY INSEDIMENTS IN JIAOJIANG RIVER ESTUARY

通过常规分离纯化、鉴定和构建细菌克隆文库的方法,研究椒江口三个站点沉积物中细菌的多样性,并对其进行系统发育分析。可培养细菌的形态学及API鉴定结果显示杀鲑气单胞菌是优势种,典型细菌16S r DNA分子鉴定结果表明γ-变形菌纲和厚壁菌门为主要类群。未培养细菌克隆文库的序列分析结果表明:细菌主要包括变形菌门、酸杆菌门、绿弯菌门、芽单胞菌门、硝化螺旋菌门、CFB群、放线菌门、厚壁菌门等8个类群;其中C0站点克隆子主要属于γ-变形菌纲及绿弯菌门;C1站点克隆子以α-变形菌纲及γ-变形菌纲为主;C2站点克隆子主要属于放线菌门及α-变形菌纲。综合可培养及未培养结果,可发现椒江口沉积物中γ-变形菌纲为典型优势类群,且相当数量的克隆子其且相当数量克隆子的相似序列来自重金属或石油烃污染的沉积环境。

We studied the diversity of bacteria in sediment from three sites in Jiaojiang River estuary and the phylogenesis in bacterial clone library in conventional isolation, purification, and identification procedures. The results of 16 S r DNA analysis showed that for cultured bacteria, Aeromonas salmonicida was the main species, and γ-Proteobacteria and Firmicutes were the dominant groups; while non-cultured bacteria included mainly Proteobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospira, CFB group, Actinobacteria, and Firmicutes. Each site featured own bacterial clone. In overall, γ-Proteobacteriain was the dominant group in sediment of the estuary, and the similar sequences of many typical clones were probably derived from environment pollution by heavy metals or petroleum hydrocarbon.