CD133和Ki-67在骨巨细胞瘤中的表达及其临床意义

Expressions of CD133 and Ki-67 in giant cell tumor of bone and their clinical significance

目的探讨CD133和Ki-67在骨巨细胞瘤(GCTB)中的表达及其与预后的关系,为临床判断其生物学行为及评估预后提供有力的证据。方法采用用免疫组织化学染色检测CD133和Ki-67蛋白在80例GCTB组织中的表达,并分析2种蛋白的表达与GCTB临床病理特征及预后的关系。结果 GCTB组织中CD133阳性表达率为43.8%(35/80),Ki-67阳性表达率为57.5%(46/80)。CD133表达与肿瘤直径、肿瘤复发、Jaffe分级、Campanacci分期及手术方式有关(P<0.05);Ki-67表达与肿瘤直径、Jaffe分级和Campanacci分期有关(P<0.05)。全组GCTB患者1、3、5年无复发生存率分别为93.0%、88.0%和80.0%。CD133阳性和阴性表达者的1、3、5年无复发生存率分别为89.0%、83.0%、75.0%和99.0%、91.0%、91.0%,差异有统计学意义(P<0.05)。Ki-67阳性和阴性表达者的1、3、5年无复发生存率分别为89.0%、75.0%、68.0%和97.0%、93.0%、90.0%,差异亦有统计学意义(P<0.05)。结论 CD133和Ki-67表达与GCTB患者的预后密切相关,是评估其恶性程度以及预后的重要指标。

Objective To investigate the expressions of CD133 and Ki-67 in patients with giant cell tumor of bone （ GCTB） and the relationship with clinicopathological features and prognosis, in order to provide firm evidence for the judgement of biological be-havior and the evaluation of prognosis. Methods Eighty cases of GCTB were analyzed and admitted to our hospital from March 2007 to March 2013. Immunohistochemical method was used to detect the expressions of CD133 and Ki-67 in the cancer tissues of patients with GCTB. The correlations of two proteins with clinicopathological features and prognosis of GCTB patients were analyzed. Results The positive expression of CD133 and Ki-67 in GCTB tissues was 43-8% （ 35/80） and 57-5%（ 46/80） , respectively. The expression of CD133 was related to tumor size, recurrence, Jaffe pathological classification, Campanacci image grade and surgical procedures（ P〈0.05） . The expression of Ki-67 was related to tumor size, Jaffe pathological classification and Campanacci image grade（ P〈0.05） . The 1-, 3-, 5-year recurrent-free survival rates were 93-0%, 88-0% and 80.0%. The 1-, 3-, 5-year recurrent-free survival rates of pa-tients with positive and negative CD133 were 89-0%, 83-0%, 75-0% versus 99-0%, 91-0%,91-0% respectively, with statistically significant difference（P〈0.05）. The 1-, 3-, 5-year recurrent-free survival rates of patients with positive and negative Ki-67 were 89-0%, 75-0%, 68-0% versus 97-0%, 93-0%, 90.0% respectively, with statistically significant difference （ P〈0.05） . Conclusion The positive expression of CD133 is closely related to the disease deterioration and recurrence of GCTB. CD133 and Ki-67 can serve as the indicator for evaluating malignant degree and prognosis of GCTB.