大黄鱼口服硝唑尼特后抗刺激隐核虫感染试验

Efficacy of Orally Administrated Nitazoxanide in Treating Cryptocaryon irritans Infectionon Pseudosciaena crocea

将硝唑尼特溶解于二甲亚砜,均匀喷于膨化饲料外涂油膜制成药物饲料,每天按鱼体重的50、100、150mg·kg-1给药,连续给药6d。用同步孵出的刺激隐核虫感染子按10 000个·尾-1鱼感染鱼体,定期检查鱼体鳃、体表和鳍上滋养体数量。150mg·kg-1组鱼体经4d给药鱼体全部死亡,50、100mg·kg-1组连续6d给药鱼体正常。刺激隐核虫感染子对试验大黄鱼能大量感染,在不同给药量组间及是否给药组间的感染差异不明显,表明大黄鱼口服硝唑尼特不能有效预防刺激隐核虫的感染。

Efficacy of nitazoxanide（NTZ）by oral administration to Pseudosciaena crocea for the Cryptocaryon irritans infection was studied.NTZ was first-dissolved in dimethyl sulfoxide to be sprayed evenly on an extruded fish meal,which was,then,coated with a thin oil film.The meal was fed to the fish every day at the rates of 50,100 or 150mg per kg of the fish body weight.The treatments lasted 6days.At the same time,simultaneously hatched theronts were introduced to infect the fish at a rate of 10 000 per P.crocea.Subsequently,the numbers of trophonts appeared on the gill,skin and fins of each fish were monitored regularly.After the 6-day treatments,all P.crocea were infected with thetheronts in large numbers,and the fish fed with 150mg·kg-1 diet died,while the others survived.The oral NTZ treatment apparently did not prevent P.croceafrom being infected by C.irritans.