多胺和植物激素对巴氏杜氏藻生长及脂类含量的影响

Influence of phytohormones and polyamines on the growth and lipid content of Dunaliella bardawil

如何提高微藻的生长速度,获取高生物量的产出,同时保持较高的脂类含量是目前微藻生物质能源研发中面临的普遍问题.本文对比研究了3种植物激素吲哚乙酸(IAA)、赤霉素(GA)和脱落酸(ABA)以及3种多胺腐胺(Put)、亚精胺(Spd)和精胺(Spm)对巴氏杜氏藻生长和脂类含量的影响.结果表明,3种植物激素和多胺对藻细胞生长均有促进作用.与对照组相比,Spm实验组的细胞密度提高了35.2%,其次为IAA,提高了26.6%.Spm处理同样提高了藻的生物量,达26.1%,而IAA则仅为9.4%.植物激素和多胺同样影响了藻细胞的脂类含量,其中Spm实验组藻细胞脂类含量最高,达29.7%,比对照组提高了22.7%,其次为IAA,提高了20.1%.因此,适量添加植物激素和多胺可有效促进巴氏杜氏藻的生长,提高脂类含量.

For mass production of bio-fuel,the economic feasibility of microalgal culture greatly depends on the productivity of biomass and lipids. The influence of 3 phytohormones（ IAA,GA,ABA） and 3 polyamines（ Put,Spd,Spm） on the growth and lipid content of Dunaliella bardawil（ HL-1） were studied. Our results show that the growth of D. bardawil is improved by phytohormone and polyamines. Cell density and biomass in Spm treatment has increased by 35. 2% and 26. 1% than that of the control,and by 26. 6% and 9. 4% in IAA treatment,comparatively. Moreover,the cell lipid content is affected by phytohormone and polyamines. The lipid content in Spm treatment is the highest（ 29. 7%） with 22. 7% increase than the control and the next is IAA treatment with 20. 1%increase. Therefore,it is useful to add phytohormone and polyamines for the growth and lipid content of D.bardawil.