GC-MS分析新疆拜城油鸡和南京土鸡鸡皮脂质中脂肪酸组成

GC-MS Analysis of Fatty Acid Composition of Lipids in Chicken Skin of Xinjiang Baicheng Fatty Chicken and Nanjing Local Chicken

分析比较新疆拜城油鸡和南京土鸡鸡皮总脂质和磷脂的含量及脂肪酸组成。用溶剂提取法分别提取2种鸡皮的总脂质和磷脂,将提取的脂质经甲酯化后,利用气相色谱-质谱分析其脂肪酸组成。结果显示,南京土鸡鸡皮总脂质含量和磷脂含量分别为35.56%和7.71%,拜城油鸡鸡皮中总脂质含量和磷脂含量分别为42.65%和8.95%,均高于南京土鸡。南京土鸡与拜城油鸡鸡皮总脂质中各含有25种脂肪酸,主要包含油酸、亚油酸、棕榈酸、硬脂酸、棕榈烯酸;2种鸡鸡皮磷脂中均含有10种脂肪酸,主要包含棕榈酸、油酸、硬脂酸和亚油酸。2种鸡的鸡皮油脂脂肪酸的种类相同,但各脂肪酸含量有所差异;脂肪酸饱和程度比例基本相似,但南京土鸡鸡皮磷脂中不饱和脂肪酸和亚油酸含量高于拜城油鸡。

In order to analyze and compare the fatty acids and phospholipids of Nanjing local chicken and Xinjiang Baicheng fatty chicken, lipids were extracted from the chicken skin by solvent extraction. The fatty acid composition of the extracted lipids was determined by gas chromatography-mass spectrometry（GC-MS） after methyl esterification. Results showed that the contents of total lipid and total phospholipid in the skin of Nanjing local chicken were 35.56% and 7.71%, respectively, and 42.65% and 8.95% in the skin of Xinjiang Baicheng fatty chicken, respectively, suggesting that total lipid and total phospholipid were significantly more abundant in Baicheng fatty chicken. Totally 25 fatty acids were identified in the skin of Nanjing local chicken and Baicheng fatty chicken by chromatographic method, respectively, mainly including 9-octadecenoic aid, 9,12-octadecadienoic acid, hexadecanoic acid, octadecanoic acid and 9-hexadecenoic acid. Ten fatty acids were common to both breeds which were dominated by hexadecanoic acid, oleic acid, octadecanoic acid and 9,12-octadecadienoic acid. Hence, the same fatty acid composition with different contents was found in the two breeds. Moreover, although the proportions of saturated fatty acids were substantially similar, the contents of unsaturated fatty acids and 9,12-octadecadienoic acid in the skin of Nanjing local chicken were higher than those of Baicheng fatty chicken.