摘要
建立分散液-液微萃取与高效液相色谱联用技术测定石斛酚的分析方法。通过对实验条件的筛选及优化,得到最佳条件：p H 3,辅以涡旋方式进行萃取,萃取溶剂为120μL正戊酸、萃取时间1 min,分散剂为曲拉通X-114、用量80μL。此方法进行萃取,检出限为2.625μg/L,定量限为8.74μg/L,线性范围为0.10~30.00 mg/L,萃取富集倍数平均值为28.02,萃取回收率平均值为1.01。将建立的分析方法应用于实际石斛样品的测定,结果表明该方法能对石斛中的石斛酚进行高效萃取与富集,方法快速简便。
A novel method for the determination of gigantol in Dendrobii Caulis by dispersive liquid-phase microextraction（DLPME） coupled with high performance liquid chromatography（HPLC） was developed. The optimal DLPME conditions were found to be extraction by vortexing for 1 min using 120 μL of valeric acid as the extraction solvent and 80 μL of Triton X-114 as the dispersant at p H 3, yielding an average 28.02-fold enrichment and an average recovery of 1.01 for gigantol. The proposed method exhibited a limit of detection（LOD） of 2.625 μg/L, a limit of quantification（LOQ） of 8.74 μg/L and a linear range of 0.10–30.00 mg/L. The method was successfully applied to analyze real samples, and the results showed that it was a rapid and efficient method for the extraction of gigantol in Dendrobii Caulis.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2015年第16期211-214,共4页
Food Science
基金
昆明学院科学研究项目(XJL12015)
