Box-Behnken响应面法优化微波提取刺五加多糖工艺

Optimization of Microwave Extraction Technology for Acanthopanax Senticosus Polysaccharide by Box-Behnken Response Surface Methodology

目的:采用微波技术提取刺五加多糖,通过Box-Behnken响应面分析法优化提取刺五加多糖工艺。方法:通过对提取工艺参数优化:料液比(X1)、微波功率(X2)和微波处理时间(X3)为考察对象,以提取率(Y)为评价指标,利用Box-Behnken效应面法优化微波提取刺五加多糖工艺。结果:刺五加多糖微波提取的最佳工艺参数为:微波提取时间22.5 min,微波功率450 W,料液比1∶25(g·ml-1),实测值与回归模型预测值的相对误差为5.68%。结论:微波提取刺五加多糖工艺采用BoxBehnken效应面法优化是简单、可行的。

Objective： To optimize the extraction conditions of microwave technology for the polysaccharides from Acanthopanax senticosus. Methods： The extraction conditions were optimized by Box-Behnken response surface methodology. The solid-liquid ratio （X1）, microwave pewer（X2 ） and extraction time（X3 ） were used as the independent variables and the extraction rate（Y） was used as the dependent variable. Results： The optimized conditions of microwave extraction technology.for Acanthopanax senticosus polysaccha- ride were as follows ： the extraction time was 22.5 min, the microwave power was 450 W, and the solid-liquid ratio was 1 ： 25 （ g · ml-1 ）. The experimental values were similar with those predicted from the regression model with the relative error of 5.68%. Conclu- sion： The Box-Behnken experimental design is simple and feasible in the optimization of the microwave extraction technology for Acan- thopanax senticosus polysaccharide.