HPLC法同时测定蛇胆追风丸中阿魏酸、蛇床子素的含量

Determination of Ferulic Acid and Osthole in Shedanzhuifeng Pills by HPLC

目的：采用HPLC法同时测定蛇胆追风丸中阿魏酸、蛇床子素的含量。方法：采用Phenomenex Synergi C18（250 mm×4.6 mm,4μm）色谱柱,流动相为甲醇-0.1%磷酸,梯度洗脱,检测波长为322 nm,流速为1.0 ml·min-1。结果：阿魏酸、蛇床子素线性范围分别为1.244～14.928μg·ml-1（r=0.999 9）、5.004～25.020μg·ml-1（r=0.999 8）,平均加样回收率分别为98.3%（RSD=1.4%,n=6）、98.2%（RSD=1.6%,n=6）。结论：该方法操作简便,结果准确可靠,可作为蛇胆追风丸中阿魏酸、蛇床子素的含量测定方法。

Objective： To establish an i-IPLC method for the determination of ferulic acid and osthole in Shedanzhuifeng pills. Methods： The analytical column was Phenomenex Synergi Cls （250 mm× 4.6 mm,4 μm）, the mobile phase was methanol-0.1% phosphoric acid with gradient elution, the UV detection wavelength was 322 nm, and the flow rate was 1.0 ml · min-1. Results： The linear range of femlie acid and osthole was 1. 244-14. 928 μg · ml - 1 （ r = 0.999 9） and 5. 004-25. 020 μg · ml -1 （ r = 0.999 8 ）, re- spectively. The average recovery was 98.3% （RSD = 1.4% ） and 98.2% （RSD = 1.6% ） （n =6）, respectively . Conclusion： The method is simple, available and accurate, which can be used to determine the content of ferulic acid and osthole in Shedanzhuifeng rills.