电针对SAMP8小鼠海马Beclin-1表达的影响

Effects of electroacupuncture on Beclin-1 expression in hippocampus of SAMP8 mice

目的从自噬角度探讨电针治疗阿尔茨海默症的作用机制。方法选取8月龄老年痴呆模型SAMP8小鼠16只,随机分为模型组8只和电针组8只,另选8月龄抗老年痴呆模型SAMR1小鼠8只为对照组,采用Morris水迷宫检测小鼠学习记忆能力,免疫组化法检测海马Beclin-1表达。结果与对照组比较,模型组平均逃避潜伏期明显延长,原平台象限停留时间缩短,海马Beclin-1表达降低(P<0.01);与模型组比较,电针组平均逃避潜伏期缩短,原平台象限停留时间延长,海马Beclin-1表达升高(P<0.05)。结论电针治疗阿尔茨海默症的机制可能是提高海马Beclin-1表达,增强自噬活性。

Objective To explore the therapeutic effect of electroacupuncture on Alzheimer＇ s disease from the point of autophagy and its mechamism.Methods Sixteen SAMP8 mice aged 8 months were randomly divided into：model group（n =8） and electroacupuncture group（n =8）.Eight SAMR1 mice aged 8 months were chosen as control group.Morris water maze was used to test mice learning and memory abilities.Beclin-1 expression was determined by immunohistochemistry.Results Compared with control group,the average escape latency was significantly lengthened in model group,and the plateau phase was shortened,and Beclin-1 expression was significantly decreased（P ＜0.01）.Compared with model group,the average escape latency significantly was shortened,the plateau phase was lengthened,and Beclin-1 expression was significantly increased in electroacupuncture group（P ＜ 0.05）.Conclusion The curative effect of electroacupuncture therapy on AD may be related to the increase of Beclin-1 expression and improvement of autophagy.