福建大花蕙兰疫病病原菌生长特性研究

Growth Characteristics of Fujian Cymbidium Blight Pathogen

对引起福建地区大花蕙兰疫病的棕榈疫霉菌进行生长特性的研究.结果表明：不同培养基对该菌生长影响不同,菌丝在V8汁培养基上生长最快,在清水琼脂培养基上生长最慢,不同培养基上菌落平均直径依次是：V8汁〉大花蕙兰叶煎汁＂番茄汁〉PDA=PSA〉 玉米粉〉 清水琼脂,最大与最小菌落平均直径差为3.23cm,且菌落形态有差异;碳源和氮源以甘露醇和牛肉膏最佳;病原菌在15～35℃,pH3～10 的条件下均能生长,最适的生长温度为30℃,最适的pH 值为6;菌丝的致死温度为50℃;光暗交替有利于该菌的生长.

Growth characteristics of Phytophthora palmivora that causes the cymbidium blight in Fujian were studied .The results showed that (a) the pathogen grew differently on different media ,(b) the bacteria grew the fastest on V8 juice ,and the slowest on water‐agar among various media , (c) the bacterial colonies formed on different media differed in size in the order of V8 juice>cymbidium leaf extract> tomato juice> PDA= PSA>corn flour> water‐agar , (d) the averaged difference between the largest and the smallest colonies was 3.23 cm in diameter ,(e) the morphology of the colonies grown on different media varied appreciably ,(f) beef extract seemed to be the best nitrogen source for the bacterial growth ,while mannitol the best for carbon ,(g) the pathogen could grow in the range of 15-35℃ and pH 3 -10 ,with the optimum temperature at 30℃and pH of 6 ,(h) the lethal temperature for the mycelia was 50℃ ,and (i) the bacteria seemed to thrive under alternated light and dark conditions .