雷尼酸锶促进大鼠股骨骨质疏松性骨折愈合的研究

Strontium ranelate promotes the fracture healing of the femurs in osteoporotic rats

目的探讨雷尼酸锶(strontium ranelate,SR)对大鼠股骨骨质疏松性骨折愈合的影响。方法 6个月龄Wistar雌性处鼠66只,切除双侧卵巢制作骨质疏松动物模型,模型建立成功后,在大鼠股骨中段横行截骨,制作大鼠股骨骨质疏松性骨折动物模型,术后死亡6只,然后将剩余60只大鼠随机分为实验组(SR干预治疗组)和空白对照组,每组30只。骨折术后第一天起,实验组给予SR 400 mg/(kg·d)灌胃,对照组给予同等体积的生理盐水灌胃。通过影像学、组织学和免疫组织化学(骨形态发生蛋白-2,BMP-2)观察,骨组织形态计量学、骨密度(bone mineral density,BMD)和生物力学测量,观察骨折愈合情况。结果实验组腰4、5椎体BMD、BMP-2阳性表达细胞数、骨小梁面积比、骨小梁平均宽度、最大负荷和最大桡度高于同时期对照组,差异有统计学意义(P<0.01);骨小梁平均间隔小于同期对照组,差异有统计学意义(P<0.01)。结论 SR具有抑制骨吸收和促进骨形成的双重作用,能增加BMD,加快骨痂形成,促进骨质疏松性骨折愈合,改善骨的显微结构,提高骨折愈合后骨的生物力学特性。

Objective To investigate the effect of strontium ranelate（ SR） on fracture healing of the femurs in osteoporotic rats.Methods Sixty-six 6-month-old female virgin Wistar rats were bilateral ovariectomized and cut the middle shaft of the femurs to create osteoporosis fracture animal model. Six rats died after surgery. The rest 60 rats were randomly divided into experimental group and control group,with 30 rats in each group. From the first day after fracture rats in experimental group received SR 400 mg /（ kg·d） by intragastric administration,and rats in control group received the same volume of normal saline. The fracture healing was observed using imaging,histology,immunohistochemistry（ bone morphogenetic protein-2,BMP-2）,bone mineral density（ BMD）,and biomechanics. Results BMD of lumbar vertebrae 4 and 5,number of BMP-2 positive cells,trabecular area,trabecular width,and the biomechanical property in experimental group were higher than those in the control group（ P〈0. 01）,and the trabecular separation in the experimental group was smaller than that in the control group（ P〈0. 01）. Conclusion SR has inhibiting effect on bone resorption and stimulating effect on bone formation. It can increase BMD,promote the osteoporosis fracture healing,improve the microstructure of the bone,and enhance the biomechanical properties.