自限性和慢性HBV感染患者IFN-ω1、IFNGR基因的单核苷酸多态性观察

Observation of gene single nucleotide polymorphisms of IFN-ω1 and IFNGR in self-limiting HBV infection and chronic HBV infection patients

目的观察自限性和慢性乙型肝炎病毒(HBV)感染患者干扰素ω1(IFN-ω1)、干扰素γ受体(IFNGR)基因的单核苷酸多态性(SNP)。方法应用聚合酶链反应-限制性片段长度多态性分析方法检测206例自限性HBV感染者(对照组)和210例慢性HBV感染者(病例组)IFN-ω1基因上4个SNP位点、IFNGR基因上4个位点,比较两组基因型和等位基因频率。结果病例组、对照组IFNGR基因的rs9376267位点CT基因型频率分别为48.4%、60.3%,两组比较,P=0.012。两组IFN-ω1、IFNGR基因其他SNP位点基因型和等位基因频率比较差异无统计学意义。结论自限性HBV感染患者IFNGR基因上的rs9376267位点CT基因型频率较慢性HBV感染患者高。

Objective To observe the gene single nucleotide polymorphisms（ SNPs） of interferon-ω1（ IFN-ω1） and interferon-γ receptor（ IFNGR） in self-limiting hepatitis B virus（ HBV） infection and chronic HBV infection patients.Methods PCR-RFLP was used to detect four SNP sites of IFN-ω1 and four sites of IFNGR in 206 cases of patients with self-limiting HBV infection（ control group） and 210 cases of patients with chronic HBV infection（ case group）. The genotype and allele frequency of the two groups were compared. Results The rs9376267 gene loci CT genotype frequencies of IFNGR in the case group and control group were respectively 48. 4% and 60. 3%（ P = 0. 012）. No statistically significant difference was found between the two groups in the genotype and allele frequency of other SNP sites of IFN-ω1 and IFNGR genes. Conclusion The CT genotype frequency on IFNGR rs9376267 site in patients with self-limiting HBV infection was higher than that of the patients with chronic HBV infection.