活血化瘀法对急性心肌梗死大鼠CD_(34)、血管内皮生长因子阳性细胞的影响

Effect of Activating Blood to Resolve Stagnation on Cells Expressing CD_(34) and Vascular Endothelial Growth Factor in Rats with Acute Myocardial Infarction

目的研究活血化瘀法对大鼠心肌组织微环境中CD34、血管内皮生长因子(VEGF)的影响,探讨其治疗急性心肌梗死的机制。方法 Sprague-Dawley大鼠32只随机分为假手术组(n=8)、模型组(n=8)、中药+粒细胞集落刺激因子(G-CSF)组(n=8)和G-CSF组(n=8)。各组均于造模3 h后给予相应药物,连续6 d,第7天处死取材。苏木素-伊红(HE)染色观察病理形态,免疫组化染色检测梗死心肌边缘区CD34、VEGF、Ki-67阳性细胞表达。结果模型组、中药+G-CSF组和G-CSF组CD34、VEGF、Ki-67阳性细胞表达均高于假手术组(P<0.05);中药+G-CSF组、G-CSF组高于模型组(P<0.05);中药+G-CSF组CD34、VEGF阳性表达高于G-CSF组(P<0.05),两组Ki-67阳性细胞表达无显著性差异(P>0.05)。结论活血化瘀法能够提高CD34和VEGF的表达,其作用优于单独使用G-CSF,可以有效治疗急性心肌梗死。

Objective To explore the effect of Activating Blood to Resolve Stagnation on the expression of CD34 and vascular endothelial growth factor（VEGF） in rats with acute myocardial infarction.Methods 32 Sprague-Dawley rats were randomly divided into sham operation group（A,n=8）,model group（B,n=8）,Xuesaitong Injection ＋ granulocyte colony-stimulating factor（G-CSF） group（C,n=8） and G-CSF group（D,n=8）.Corresponding medicine was given to each group 3 hours after modeling,for 6 days.Pathomorphological changes were observed through HE staining,and the expression of CD34,VEGF and Ki-67 were observed through immunohistochemical staining.Results The expressions of CD34,VEGF and Ki-67 were higher in groups B,C and D than in group A（P〈0.05）,and were higher in group groups C and D than in group B（P〈0.05）.The expressions of CD34 and VEGF were higher in group C than in group D（P〈0.05）.However,there was no significant difference in the expression of Ki-67 between 2 groups（P〉0.05）.Conclusion The expression of CD34 and VEGF increases with Activating Blood to Resolve Stagnation method,which is superior to using G-CSF only.Activating Blood to Resolve Stagnation may play an important role in the treatment of acute myocardial infarction.