不同贮藏条件下棉花叶片DNA提取效果分析

Analysis on DNA Extraction Effect from Cotton Leaves under Different Storage Conditions

针对棉叶贮藏方法不恰当导致DNA提取得率及纯度较差的问题,本研究以新鲜棉叶为对照,比较了5种贮藏条件下的棉叶DNA提取效果,以期筛选出一种棉叶贮藏的较优模式,从而提高DNA的提取得率及纯度。结果表明,-20℃冷冻3 d的棉叶DNA得率最高且纯度较好,甚至比新鲜叶片的DNA得率高51.4%;3 d冷冻贮藏的DNA得率明显高于3 d冷鲜贮藏的;7 d液氮冷冻贮藏的DNA得率明显优于7 d硅胶干燥贮藏的和冷冻贮藏的;7 d液氮速冻贮藏的DNA纯度与7 d硅胶干燥贮藏的相近。综上所述,短期贮藏可采用-20℃冷冻保存的方法;较长时间的保存建议采用液氮速冻的贮藏方式。

This study was conducted based on the problems that inappropriate storage could lead to poor extraction rate and purity of DNA from cotton leaves. With the fresh leaves as control,the DNA extraction effects of cotton leaves were compared after storage in five kinds of conditions to screen out a best storage method. The results showed that the DNA extraction rate of cotton leaves was the highest after- 20℃ frozen storage for 3 days with better purity,which increased by 51. 4% compared to that of the control. The DNA yield of 3- day frozen storage was obviously higher than that of 3- day 4℃ storage,and the 7- day liquid nitrogen frozen had clear advantages over the silica gel dry storage and 7- day frozen storage. The DNA purity of 7- day liquid nitrogen frozen was close to that of 7- day silica gel dry storage. In conclusion,- 20℃ frozen storage could be used for short- term storage,while the liquid nitrogen frozen could be used for longer- time storage.