急性酒精性肝损伤小鼠模型及早期诊断指标GSTA1的研究

Study on GSTA1,an early indicator of alcohol induced acute liver injury in mice

为研究谷胱甘肽S转移酶A1(GSTA1)在肝损伤早期诊断中的价值,本试验通过检测血清谷丙转氨酶(GPT)及肝组织指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)变化,成功复制急性酒精性肝损伤小鼠模型;通过时间-效应和剂量-效应关系研究血清中GSTA1和ALT变化。结果显示,给予小鼠灌服乙醇溶液后,2 h血清中GSTA1含量变化与对照组相比差异极显著(P<0.01),而ALT活性变化在6 h才显示出一定的差异性(P<0.05);当乙醇剂量为10 m L/kg体重时,血清中GSTA1含量变化与对照组相比差异极显著(P<0.01),而当乙醇剂量达到14 m L/kg体重时,ALT活性变化与对照组相比差异极显著(P<0.01)。表明在急性酒精性肝损伤模型中,GSTA1的含量变化在肝损伤早期就可检测出来,作为肝损伤标记物,GSTA1比ALT更敏感。

To study the value of GSTA1 as an early indicator,through detecting the changes of ALT in serum and MDA,SOD,GSH and GSH-px in liver tissue,the acute alcoholic liver injury mice model was successfully replicated.GSTA1 and ALT changes were analyzed by time-response and dose-response studies.The results showed that significant increases in GSTA1 were detected at 2 h,but showed significant difference at 6 h after alcohol exposure.GSTA1 release was significantly increased at a dose of 10 m L/kg,release of ALT did not significantly increase until the administration of a dose of 14 m L/kg.These results suggested that the variation of GSTA1 could be detected during the early stage of acute alcoholic liver injury,GSTA1 was a more sensitive indicator than ALT.