尼莫地平对大鼠急性脑缺血再灌注损伤NF-κB和caspase-3蛋白表达的影响

Effects of nimotop on NF-κB and caspase-3 expression in rat brain tissue after cerebral ischemic reperfusion

目的探讨尼莫地平对大鼠急性脑缺血再灌注损伤中NF-κB和caspase-3蛋白表达的影响。方法 45只雄性SD大鼠随机分为3组,即假手术组、模型组和尼莫地平注射液治疗组,每组15只。参照Zealonga等的改良线栓法制备大鼠脑缺血再灌注模型。脑缺血再灌注后,利用Bederson法评价各组大鼠神经功能评分,采用TUNEL法检测大鼠海马CAI区神经元凋亡率,免疫组织化学和Western blot法检测NF-κB和caspase-3蛋白表达情况。结果脑缺血再灌注24h后,模型组NF-κB和caspase-3表达均高于假手术组,差异有统计学意义(P<0.05);与模型组相比较,尼莫地平治疗组NF-κB和caspase-3的表达显著降低,差异有统计学意义(P<0.05)。结论尼莫地平可能通过抑制NF-κB和caspase-3的蛋白表达,对缺血再灌注损伤起到保护作用。

Objective To investigate the effect of nimotop on the expression of NF-κB and caspase-3 in the hippocampus CAI regions of rats with cerebral ischemic reperfusion. Methods 45 male Sprague-Dawley rats were randomly divided into sham-operated group,model group and nimotop treatment group,each had 15 rats. The rat model of middle cerebral artery occlussion（ MCAO） were established according to Zealonga method. After 24 h cerebral ischemia reperfusion,Bederson method was used to evaluate nerve function score,TUNEL method to detect the rat hippocampal CAI area neuronal apoptosis rate,immunohistochemistry and Western blot to detect NF-κB and caspase-3 protein expression. Results After 24 h cerebral ischemia reperfusion,compared with control group,NF-κB and caspase-3 protein expression in model group were increased,the difference between two group was significant（ P〈0. 05）; compared with model group,NF-κB and caspase-3 protein expression in nimotop treatment group were significantly reduced,and the difference was significant（ P〈0. 05）. Conclusion Nimotop has protective effect to ischemia-reperfusion injury rats,its mechanism may be related to inhibit the NF-κB and caspase-3 protein expression.