正交试验筛选北五味子保肝活性成分的提取工艺研究

Study on extraction process of screening hepatoprotective activity component of Schisandra chinensis by orthogonal test

目的筛选北五味子中3种保肝活性成分的最佳提取工艺。方法分别采用超声法和乙醇回流提取法,根据正交试验,提取北五味子中的3种保肝活性成分,应用反相高效液相色谱法,测定北五味子中3种保肝活性成分的含量。结果以北五味子中3种保肝活性成分的含量为指标,最佳提取条件为85%乙醇回流提取3 h、料液比例1∶8、提取次数2次。结论该提取方法设计科学合理,提取工艺简单,提取率高,重现性好,适于工业生产,为研究北五味子的保肝药物新剂型和药材合理应用提供参考。

Objective To select out the optimal extraction process of three liver protective compounds of Schisandra chinensis. Methods The L9（ 34） orthogonal experimental design was adopted,the extraction time,material： liquid ratio,ethanol concentration,and extraction times as the factors to determine the optimum conditions for detecting three liver protective compounds of Schisandra chinensis by ultrasonic method and reflux extraction with reversed-phase high performance liquid chromatography（ RP-HPLC）. Results The optimum extraction process was 85% ethanol concentration,8 ×solvent consumption,3 hours of extraction time,twice of extraction times. Conclusion Optimizing the extraction process of three liver protective compounds of Schisandra chinensis can provide a reference for further research of three liver protective compounds of Schisandra chinensis.