赤霉素和多效唑对红花生长和脂肪酸组成的影响

Effects of gibberellin acid( GA) and paclobutrazol( PP_(333))utilization on growth and fatty acids of safflower

为研究激素对红花生长及脂肪酸合成的影响,采用赤霉素（30、100和170mg/L）及其生物合成抑制剂——多效唑（30、90和150mg/L）对伸长期红花进行喷施处理,考察其部分农艺性状,并采用GC-MS测定其花与种子脂肪酸组成和含量。结果表明,赤霉素可显著增加红花株高和分枝长,降低其单株总果球数和一级分枝数,并使始花期提前1～4d。使用赤霉素可显著提高开花后第2d花器官的亚油酸和亚麻酸含量。其中,30mg/L赤霉素使红花种子亚油酸含量达67.83%,显著高于对照（62.58%）。多效唑显著降低分枝长,使始花期延迟1～2d,对其它农艺性状影响均不显著。150mg/L多效唑使红花种子油酸含量达到20.05%,显著高于对照（15.24%）,并显著提高种子（花后20d）脂肪酸O/L值。说明可采用30mg/L赤霉素提高红花种子中亚油酸含量,用150mg/L多效唑提高种子中油酸含量。

To regulate safflower growth and seeds quality,hormone effects were investigated by using GA（ 30,100,170 mg / L） and its biosynthesis inhibitor PP333（ 30,90,150 mg /L）. Hormones were sprayed at elongation stage. Several agronomic traits were measured and fatty acid in vegetative organs and seeds were analyzed by gas chromatography / mass spectrometry（ GC- MS）. Results showed that GA increased plant height and branch length significantly,and decreased primary branches and total capitulums per plant. Initial flowering date was delayed for1- 4 days. GA spray significantly increased flower linoleic and linolenic acid contents. GA（ 30 mg / L） increased seed linoleic acid to 67. 83%,which was significantly higher than that of water control（ 62. 58%）. PP333 significantly decreased branch length,delayed the initial flowering date for 1- 2 days with little effects on other traits.PP333（ 150 mg /L） increased seed oleic acid to 20. 05%,which was significantly higher than that of control（ 15. 24%）. It also remarkably increased the seed O/L at 20 d after flowering. These results indicated that 30 mg /L GA could be used to improve seed linoleic acid,and 150 mg / L PP333 to improve oleic acid.