摘要
为满足掺伪识别要求,采用固相萃取分离富集食用油中大豆苷元,优化液相色谱质谱条件,建立食用油中大豆苷元液相色谱串联质谱的检测方法。该方法对大豆苷元检测的线性方程为y=8544.77x＋58269.4,相关系数为0.9985,回收率为97.6%～117.2%,相对标准偏差小于8.3%,方法检出限为0.02μg/kg,定量限为0.06μg/kg。利用建立的大豆苷元检测方法检测植物油大豆苷元含量,结果表明仅大豆油含有大豆苷元,在花生油、菜籽油和芝麻油中未检出大豆苷元,该检测技术能够对植物油大豆苷元成分定量检测并可进行保真掺伪鉴别,为保证植物油质量、安全消费提供关键技术支撑。
A method was developed for determination of daidzein in vegetable oil by solid phase extraction-high performance liquid chromatography（ HPLC） tandem mass spectrometry. The extraction procedure and liquid chromatography tandem mass spectrometry conditions were optimized. The linear regress equation was y = 8 544. 77x＋ 58 269. 4,with correlation coefficient of 0. 998 5. The limit of detection and limit of quantification were 0. 02μg /kg and 0. 06μg / kg,respectively. The recoveries of daidzein in oil samples were in the range of 97. 6- 117. 2%with RSD less than 8. 3%. The results showed that only soybean oil contained daidzein. The proposed method could be used in detecting daidzein and adulteration in vegetable oil,which further provide critical technical supports to ensure the vegetable oil quality and consumer security.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2015年第4期561-565,共5页
Chinese Journal of Oil Crop Sciences
基金
国家科技支撑计划(2012BAK08B03)
国家自然科学基金(31201447
21205118)
"双打"相关产品检验鉴定方法研究专项(2012104010-4)
关键词
固相萃取法
液相色谱串联质谱
大豆苷元
植物油
Solid phase extraction
High performance liquid chromatography（HPLC）-tandem mass spectrometry
Daidzein
Vegetable oil
