摘要
目的研究复心汤对阿霉素所致心力衰竭大鼠心肌肥厚模型中PI3K-Akt-GSK3β通路在心肌中表达的影响,探讨复心汤对抗心力衰竭的作用机制。方法健康成年的雄性Wistar大鼠75只,随机分为空白组、模型组、卡托普利组、复心汤低剂量组、复心汤高剂量组,除空白组外,其余各组用阿霉素造模后,给予相应干预措施。4周后分别用免疫组化、Western blotting法检测心肌中PI3K、Akt及GSK3β的表达情况,并进行定量分析。结果模型组PI3K、Akt阳性表达面积最高,复心汤低剂量组、卡托普利组、复心汤高剂量组较低,空白组最低;GSK3β蛋白表达情况最高为空白组,卡托普利组、复心汤高剂量组、复心汤低剂量组表达面积较模型组高。结论复心汤治疗心力衰竭与PI3K-Akt-GSK3β通路有关。
Objective To study ＂Fuxin Decotion＂ on the expression of PI3K-Akt-GSK3β pathway in cardiac failure rats with cardiac muscle hypertrophy induced by adriamycin, and to study the action mechanism of ＂Fuxin Decotion＂ on the effect of the cardiac muscle in heart failure. Methods 75 healthy adult male Wistar rats were randomly divided into blank group, model group, captopril group, ＂Fuxin Decotion＂ low-dose group, ＂Fuxin Decotion＂ high-dose group. Each group except blank group was treated respectively after modeling with adriamycin. Expression and quantitative analysis of PI3K, Akt and GSK3β of myocardial were detected by methods of immunohistochemistry and Western blotting respectively after 4 weeks. Results PI3K, Akt positive expression area in model group was the highest, captopril group, ＂Fuxin Deeotion＂ low-dose group and ＂Fuxin Deeotion＂ high-dose group was lower, and the blank group was the Lowest; the GSK3β protein expression in the blank control group was the highest compared with captopril group, ＂Fuxin Decotion＂ low-dose group and ＂Fuxin Decotion＂ high- dose group. Conclusion ＂Fuxin Decotion＂ in treating heart failure is related to PI3K-Akt-GSK3β pathway.
出处
《上海中医药杂志》
2015年第8期74-78,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81273703)
