培养方式对蛹虫草液体发酵产核苷物质的影响

Effects of Culture Conditions on Nucleoside Production by the Medicinal Fungus Cordyceps militaris

本文考察了蛹虫草CM3,08Y1菌株在通过光照振荡、光照静置、黑暗振荡、黑暗静置四种培养方式和添加前体物质(腺嘌呤1 g/L+甘氨酸16 g/L),发酵60 d后,发酵液内虫草素和腺嘌呤含量变化。结果表明:CM3菌株在静止培养及添加前体时虫草素的含量达2 541.06 mg/L,腺嘌呤利用率达99.79%;08Y1菌株在光照静止培养及添加前体时虫草素的含量达2 875.71 mg/L,腺嘌呤利用率达99.75%,这为蛹虫草高产虫草素提供了有效的方法,同时说明不同菌株在不同条件下,其代谢产物的量具有明显的差异。

In order to analyze the effects of additives（ adenine 1 g / L ＋ glycine 16 g / L） and different culture conditions（ light and shaking,light and static,dark and shaking,dark and static） on cordycepin production and utilization ratio of adenine,strain of C. militaris 08Y1 and CM3 were investigated in this paner. The maximum cordycepin production of 2 541. 06 mg / L was achieved by culture at light and static with the supplement of additives by strain of C. militaris CM3,and the maximum utilization ratio of adenine reached to 99. 79 %. At the same time,cordycepin production reached to 2 875. 71 mg / L at light and shaking with the supplement of additives by the strain of C. militaris CM3,and the maximum utilization ratio of adenine reached to 99. 75 %. The results show that there are some differences for the production of metabolite in different culture conditions and strains.