人畜粪便隐孢子虫快速检测方法研究

Research on the detection methods of Cryptosporidium for human and animal feces

目的探求RIDA隐孢子虫快速检测法检测人和牲畜粪便隐孢子虫的优缺点,验证该方法与金胺-酚改良抗酸染色法检测结果的一致性。方法随机采集200位人的粪便和72头牲畜的粪便分别用RIDA隐孢子虫快速检测法和金胺酚-改良抗酸复染法检测隐孢子虫;用SPSS对两种检测方法的结果进行卡方检验。结果 RIDA隐孢子虫快速检测法检测出儿童和牲畜粪便的阳性率分别为5.00%和13.89%;用金胺酚-改良抗酸复染法检测出的人和牲畜粪便的阳性率分别为4.50%和25.00%;两种方法检测人类粪便的结果差异性无统计学意义(P>0.05);但两种方法牲畜粪便的检测结果差异有统计学意义(P<0.05)。结论 RIDA隐孢子虫快速检测法适用于检测大样本的人类粪便隐孢子虫,并具有较好的效果。

Objective To know the advantages and disadvantages of RIDAQuick Cryptosporidium method for the Cryptosporidium detection among feces of human and live stocks,and find out the consistency to auramine-phenol modified acid-fast staining method.Methods 200 and 72 feces of human and live stocks were separately collected. All feces were detected by RIDAQuick Cryptosporidium method and auramine-phenol modified acid-fast staining. Chi-square test was used to analyze the results of the two detection methods by SPSS. Results The positive rates of people and live stocks using RIDAQuick Cryptosporidium were 5% and13. 9%,respectively. And the positive rates of human and live stocks using auramine-phenol modified acid-fast staining method were4. 5% and 25%,respectively. Two methods for human feces detection were of no significant difference（ P〈0. 05）,however,there were significant differences between two methods for the detection of livestock feces（ P〈0. 05）. Conclusions RIDAQuick Cryptosporidium method had good results for Cryptosporidium detection in a large sample of human feces.