豆浆和豆粕中提取蛋白质组分7S与11S的SDS-PAGE分析

Analysis on the 11S and 7S components of protein extracted from soymilk and soy meal under SDS-PAGE

为了给予消费者在食用豆浆和豆粕时有益的参考,以普通市售黑豆、黄豆和青豆为原料,通过豆浆机(JYDZ-56)制备豆浆和豆粕,用"碱溶酸沉"法提取豆浆和豆粕中的蛋白质并进行SDS-PAGE,按照Liu S.H.提出的方法经Quantity One分析获得蛋白质组分及其相对含量。结果表明,(1)从黑豆、黄豆和青豆的豆浆中分别提取的蛋白组分均以11S为主,7S为辅,其11S组分相对含量分别为36.67%、14.94%和22.01%;11S/7S比值分别为11.04、6.31和7.24。(2)11S组分中以11S-1亚基组相对含量最高,黑豆、黄豆和青豆中的11S-1占11S组分相对含量分别为64.7%、63.3%和54.2%。(3)从黑豆、黄豆和青豆的豆粕中提取的11S和7S组分及其相对含量差异很大。黄豆豆粕的11S和7S组分分别含有3个亚基组,11S/7S比值为4.3。青豆豆粕中没有7S组分,而11S组分只有1个亚基且相对含量很低。黑豆豆粕的7S组分只有一个亚基且相对含量很低,11S组分含有2个亚基且相对含量也很低。

The 11S and 7S components and their relative content of protein extracted by the method of alkali-solution and acid-isolation extraction from soymilk and soy meal, which were made by the soymilk machine （JYDZ-56） with black soybean, soybean and green soybean as materials, were studied according to Liu S. H. method with Quantity One under SDS-PAGE, in order to providing useful reference to consumers of soymilk and soy meal. The results showed that （1） the most of protein extracted from the soymilk of black soybean, soybean and green soybean, respectively, were 11S. A little of them were 7S. The relative content of their 11S were 36.67%, 14.94% and 22.01%, respectively. The ratio 11S/7S of them were 11.04, 6.31 and 7.24, respectively. （2） The relative content of subunit group of 11S-1 was the highest in 11S. The percent of 11S-1 in 11S relative content in the soymilk of black soybean, soybean and green soybean were 64.7, 63.3 and 54.2, respectively. （3） There existed more different in 11S and 7S components and their relative content of protein extracted from the soy meal of black soybean, soybean and green soybean, respectively. Three subunit groups were included in 11S and 7S of soy meal of yellow soybean, respectively. Its ratio 11S/7S was 4.3. There existed only one subunit of 11S with low relative content, but without 7S in the soy meal of green soybean. There existed one subunit of 7S, two subunits of 11S with low relative content in the soy meal of black soybean.