电针不同穴位对慢性应激抑郁大鼠的抗抑郁作用及对血浆胃饥饿素的影响

Effect of Electroacupuncture Stimulation of“Baihui”(GV 20),etc.on Changes of Behavior and Plasma Ghrelin Content in Depression Rats

目的:观察针刺不同穴位对慢性轻度不可预知应激(UCMS)抑郁模型大鼠的抑郁样行为及血浆中胃饥饿素(ghrelin)含量的影响。方法:45只SD大鼠随机分正常组、模型组、电针1组、电针2组和氯丙咪嗪组。采用UCMS复制抑郁模型。电针1组针刺"百会"+"安眠"穴、电针2组针刺"百会"+"足三里"穴,隔日电针治疗30min;氯丙咪嗪组每天腹腔注射5mg/kg氯丙咪嗪均治疗4周。在UCMS 4周及8周时进行强迫游泳实验、旷场实验和蔗糖偏好实验;血浆ghrelin的含量采用酶联免疫吸附法进行检测。结果:UCMS刺激4周后,模型组、电针1组、电针2组和氯丙咪嗪组在强迫游泳实验中的静止时间均显著增加,挣扎时间显著降低(P<0.05,P<0.01),表现出显著的绝望行为;在旷场实验中的直立次数和总路程差异无统计学意义(P>0.05),说明活动度无差异。各治疗组治疗4周后,绝望行为得到显著改善(P<0.05,P<0.01);蔗糖偏好率均显著高于模型组(P<0.05,P<0.01),改善了应激大鼠的快感缺失。UCMS 8周后各组大鼠血浆中ghrelin的含量差异无统计学意义(P>0.05)。结论:电针"百会"穴+"安眠"穴、"百会"穴+"足三里"穴均可以显著改善UCMS抑郁模型大鼠的抑郁样行为,且两种不同穴位的配伍疗效并无显著差异;抑郁样行为后期的变化(恶化或改善)与血浆中ghrelin含量无直接联系。

Objective To observe the effect of electroacupuncture（EA）stimulation of＂Baihui＂（GV 20）＋＂Anmian＂（EX-HN 16）and＂Baihui＂（GV 20）＋＂Zusanli＂（ST 36）on behavior reactions and plasma ghrelin level in depression rats,so as to explore the correlation between its antidepressant effect and plasma ghrelin level.Methods A total of 45 SD rats were randomly divided into 5groups：normal control,model,Baihui（GV 20）＋Anmian（EX-HN 16）,Baihui（GV 20）＋Zusanli（ST 36）and medication（clomipramine）groups,with 9rats in each group.The depression model（unpredictable chronic mild stresses,UCMS）was established by giving the animals with higher temperature environment（45 ℃,5min）,forced ice-water swimming（0-4 ℃,5min）,day and night reversal environment（12h）,stroboflash stimulation（12h）,noisy stimulation（12h）,rocking-bed movement（30min）and damp pad dwelling（6-24h）,etc.for 4weeks.EA was applied to GV 20-EX-HN 16,and GV 20-ST 36 for 30min once every other day for 4weeks after modeling.For rats of the medication group,clomipramine（5mg/kg）was given（i.p.）once a day for 4weeks after modeling.The forced swimming test,sucrose preference test and open field test were used to evaluate the rats＇ depressive-like behavior.Plasma ghrelin content was assayed by ELISA.Results After exposure to UCMS for4 weeks,the immobility time was significantly increased,and the struggling time was significantly decreased in the model group（P〈0.05,P〈0.01）.In comparison with the model group,the immobility time levels were obviously decreased,while the struggling time and sucrose preference were markedly increased in the Baihui（GV 20）＋Anmian（EX-HN 16）,Baihui（GV 20）＋Zusanli（ST 36）and medication groups（P〈0.05,P〈0.01）.No significant changes were found in the rearing times and total distance of open-field test（locomotor activity）and plasma ghrelin content among the 5groups among all the groups（P〈0.05）.No significant differences were found among the two EA and medication groups in the decreased immobility time and the increased struggling times and sucrose preference levels（P〉0.05）.Conclusion EA intervention can improve the depression rats＇ hopeless behavior of forced swimming test and anhedonia behavior（sucrose preference test）,which may be not correlated to plasma ghrelin level at the late-stages and the antidepressant effect of EA intervention.