三种黑籽南瓜RAPD分析

RAPD Analysis on Three Cucurbita ficifotia

以"绿皮"、"白皮"、"绿皮花纹"3种云南黑籽南瓜为试验材料,采用RAPD技术分析了3种类型黑籽南瓜间的亲缘关系。结果表明:从38种随机引物中筛选出20种引物,选取扩增清晰、重复性好的6种引物分析,共产生43条谱带,多态谱带39条,多态性位点比率为90.70%;"绿皮"、"白皮"和"绿皮花纹"之间的平均遗传距离是0.94,"白皮"和"绿皮花纹"间的平均遗传距离为0.40;"绿皮"与"白皮"及"绿皮花纹"间的亲缘关系较远,而"白皮"与"绿皮花纹"间的亲缘关系较近。

The genomic DNA of 3 Cucurbita ficifotia whose names were‘Green peel＇,‘White peel＇and‘Green peel with White peel＇respectively were extracted by CTAB and were used to analysis the genetic relationships by RAPD molecular markers.RAPD was affected by many factors,so this experiment was for improved RAPD method.5factors and 4levels orthogonal design and screening of RAPD-PCR reaction system was used,the three kinds of black seed pumpkin relatedness were analyzed.The results showed that from 38 kinds of random primers selected 20 kinds of primers,6kinds of the primers＇ analysis was clear amplification,and better repeatability.Total 43 bands were amplified,in which 39 bands were polymorphic.The percentage of polymorphic band was 90.70%.The cluster analysis results were indicated as follows：the average genetic distance among‘Green peel＇,‘White peel＇and‘Green peel with white spot＇was 0.94.The genetic distance between‘White peel＇and‘green peel with white spot＇was 0.40.The results showed that the Cucurbita ficifotiavarieties of relationships：‘Green peel＇and‘White peel＇,‘Green peel with white spot＇had far more distant relatives,and then‘White peel＇and‘Green peel with white spot＇had close genetic relationship.