远程缺血后适应中大鼠海马CA1区TLR4和IL-6的表达变化及其意义

Expression and significance of changes of TLR4 and IL-6 in hippocampal CA1 area of rats after ischemia adaptation in distance

目的:观察大鼠远程缺血后适应时海马CA1区TLR4、IL-6表达变化并探讨其意义。方法:72只雄性SD大鼠随机分为假手术组、对照组、远程缺血后适应组,各组又分为12 h、24 h、48 h、72 h各时间点组,每组6只。应用改良Longa法建立大鼠脑缺血再灌注损伤模型并以此为对照组,在再灌注即刻用止血带结扎/放松双后肢根部各30 min循环3次,作为远程缺血后适应处理方法。HE染色观察大鼠海马CA1区组织病理学变化,免疫组织化学染色观察大鼠海马CA1区TLR4和IL-6的表达。结果:在大鼠海马CA1区,远程缺血后适应组神经元缺失、细胞肿胀较对照组明显减轻。与假手术组相比,对照组与远程缺血后适应组TLR4和IL-6表达明显增高(P<0.05);且远程缺血后适应组TLR4和IL-6的表达较对照组明显减少(P<0.05)。结论:远程缺血后适应具有脑保护作用,这种作用可能与抑制TLR4和IL-6表达有关。

Objective： To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning（ RIP） and explore its significance. Methods： All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly. Each group was divided into 4 time points： 12 h,24 h,48 h and 72 h group. There were 6 rats in each group. Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group. The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min,repeat 3 times. To observe the pathological variation of hippocampus CA1 region by HE dyeing; to test the expression of TLR4 and IL-6 by immunohistochemical staining. Results： Compared to contrast group,neuronal loss and swelling reduced significantly in RIP group. Compared to sham group,the TLR4 and IL-6 expression in contrast group and RIP group increased significantly（ P〈0. 05）. Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly（ P〈0. 05）. Conclusion： RIP dose have protective effect on cerebral ischemia. The effect may be associated with the inhibition of TLR4 and IL-6 expression.