玉米秸秆与猪粪混合发酵产沼气及其细菌解析

BIOGAS PRODUCTION AND ITS BACTERIA COMMUNITY DURING CO-FERMENTATION OF PIG MANURE AND CORN STALKS

以总固体1：1的玉米秸秆和猪粪为混合原料，采用10L厌氧反应器进行批式全混中温沼气发酵，并利用454焦磷酸测序法测定混合发酵过程中细菌群落变化。结果表明：预处理玉米秸秆和猪粪混合沼气发酵启动迅速，在笫4天达到产气高峰，容积产气率为1．17L／（L·d），46d原料沼气产率为356．55mL／gVS。该混合发酵系统中共有23～27门细菌，其中优势菌群为拟杆菌门（Bacteroidetes）、厚壁菌门（Firmicutes）和变形菌门（Proteobacteria）。发酵期内的Shannon指数在4．88～5．53之间，说明发酵期内细菌多样性比较稳定。

Using pretreated corn stalks and pig manure （total solid ratio 1 ： 1 ） as mixed materials, 10 L anaerobic reactor was used to carry out the mesophilic batch co-fermentation for biogas production;The 454 pyrosequencing was used to measure change of bacteria community in mixed fermentation process. The results show that the start-up of the co- fermentation of pretreated corn stalks and pig manure is very quickly. The daily biogas yield reached the maximum of 1.17 L/（L· d） in 4th day. The biogas yield was 356.55 mL/g VS in 46 days. There were 23-27 different bacteria Phylum in the co-fermentation system and the dominant phyla were Bacteroidetes, Firmicutes and Proteobacteria among them. The Shannon index during the co-fermentation process was between 4.88-5.53. It indicated the diversity of bacteria was relatively stable in co-fermentation period.