Tau蛋白和磷酸化Tau蛋白在C57BL/6小鼠和转基因鼠的表达

Expression of Tau protein and phosphorylation Tau protein in C57BL/6 and transgenic mice

目的探讨总Tau蛋白(T-tau)和磷酸化Tau蛋白(P-tau)在慢性青光眼模型小鼠视神经中的表达以及意义。方法选取C57BL/6雄性小鼠以及APP+转基因鼠各40只,每只小鼠右眼为实验眼,左眼为对照眼,通过烙闭实验眼巩膜上静脉建立慢性青光眼模型,采用免疫组织化学方法,检测视神经T-tau以及P-tau在不同青光眼模型中的表达情况。结果 C57BL/6小鼠以及APP+转基因鼠实验眼术后眼压均高于术前(均为P<0.05),而两组术后眼压差异无统计学意义(P>0.05)。C57BL/6小鼠以及APP+转基因鼠实验眼T-tau IOD值均低于对照眼(均为P<0.05),而P-tau IOD值均高于对照眼(均为P<0.05);C57BL/6小鼠实验眼T-tau和P-tau IOD值分别为102.24±12.12和172.12±9.01,均小于APP+转基因鼠(均为P<0.05)。APP+转基因鼠实验眼视神经细胞凋亡率为(9.72±1.08)%,明显高于C57BL/6小鼠实验眼(7.24±1.22)%,差异有统计学意义(均为P<0.05),且两者实验眼均高于对照眼(均为P<0.05)。结论慢性青光眼小鼠视网膜T-tau蛋白表达量降低,Tau蛋白磷酸化水平增加,视神经细胞凋亡率上升,在APP+转基因小鼠中尤为明显。

Objective To investigate the expression of total Tau protein（T-tau）and phosphorylation Tau protein（P-tau） in optic nerve of chronic glaucoma mice model and its significance.Methods Forty C57BL/6 mice and APP^＋ transgenic mice were chosen,the right eye of each mouse as the experimental eye,the left eye as control,the chronic glaucoma model was established by ligating four episcleral vein in the experimental eye,and the immunohistochemical staining method was used to detect the expression of T-tau and P-tau in the optic nerve.Results The postoperative intraocular pressure in the experimental eyes of C57BL/6 mice and APP^＋ transgenic mice were higher than those before operation（all P〈0.05）,while the difference in the postoperative intraocular pressure between C57BL/6 mice and APP^＋ transgenic mice was not statistical significant（P〈0.05）.IOD value of T-tau protein in the experimental eyes of C57BL/6 mice and APP^＋ transgenic mice were lower than that in the control eyes（all P〈0.05）,while the IOD value of P-tau protein were higher than the control eyes（all P〉0.05）;IOD values of T-tau and P-tau protein in the experimental eyes of C57BL/6 mouse were 102.24±12.12 and 172.12±9.01,respectively,were less than APP^＋ transgenic mice（all P〈0.05）.The apoptotic rate of optic nerve cell in the experimental eyes of APP^＋ transgenic mice was（9.72±1.08）%,significantly higher than that of C57BL/6 mice（7.24±1.22）%,the difference was statistically significant（P〈0.05）,and the experimental eyes were all higher than the control eye（all P〈0.05）.Conclusion The expression of T-tau protein in retina of chronic glaucoma mice decreases,Tau protein phosphorylation level increases,apoptotic rate of optic nerve cell increases,especially in APP^＋ transgenic mice.