异硫氰酸酯水平与肺癌关系病例对照研究

Association of urine isothiocyanate ester levels with lung cancer: a casecontrol study

目的探讨异硫氰酸酯（ITC）水平与肺癌的关系，为肺癌的防治提供参考依据。方法采用以医院为基础病例对照研究方法对河南省南阳市中心医院2012年5月—2014年11月收治的88例肺癌患者及同期在该医院进行健康体检的84名健康体检者进行问卷调查，并采用高效液相色谱法检测人尿液中ITC代谢量。结果病例组患者十字花科蔬菜摄人量、膳食中ITC摄入量和尿液中1TC代谢量分别为（蛆7±6．4）g／d、（11．7±1．0）gmol／d和（0.93±0.2）μmol／g，均低于对照组体检者的（105．6±8．9）g／d、（12．6±1．2）μmol／d和（1．63±n3）μmol／g，差异均有统计学意义（均P〈Q05）；多因素logistic回归分析结果显示，在调整了性别、年龄、体质指数、肺癌家族史及吸烟、饮酒、饮茶情况等混杂因素后，膳食中ITC摄入量≥15．305μmol／d者患肺癌风险为ITC摄入量〈9．173μmol／d者的0．70倍（OR=0．70，95％CI=0．51—0．96），尿中ITC代谢量0．825—3．342和≥3．343μmol／g者患肺癌风险分别为ITC代谢量〈0．825μmol／g者的0．68倍（OR=0．68，95％CI=0．50～0．93）和0．48倍（OR=0．48，95％CI=0．32～0．72）。结论增加日常饮食中十字花科蔬菜的摄入量可能会将低肺癌的患病风险。

Objective To explore the relationship between urinary isothiocyanates （ITCs） level and incidence of lung cancer. Methods A case-control study was conducted among 88 lung cancer patients and 84 health physical exami- nees in Nanyang Municipal Central Hospital from May 2012 through November 2014, with a face-to-face survey using a self-designed questionnaire and a food frequency questionnaire. The urinary levels of isothiocvanates of the participants were measured with high performance liquid chromatography（HPLC）. Results The levels of cruciferous vegetables in- take, ITC dietary intake, and urinary ITC were 94.7 ± 6.4 g/day, 11. 7 ± 1.0 μmol/day, and 0. 93± 0. 2 μmol/g in the cases, respectively, lower than those （105.6 ± 8.9 g/day, 12. 6 ± 1.2 μmol/day, and 1.63 ± 0. 3 μmol/g） in the controls （ all P 〈 0. 05 ）. Multivariate logistic regression analysis showed that, after adjusting confounding factors such as gender, age, body mass index, family history of lung cancer, smoking, and tea drinking, the odd ratio of lung cancer for the partici- pants with a ITC dietary intake of ≥ 15. 305 μmol/day was 0. 70（95% confidence interval[95% CI] ：0. 51 -0. 96） compared to those with the intake of 〈9. 173 μmol/day;the odd ratios of lung cancer for the participants with the urinary ITC metabolism levels of 0. 825 -3. 342 μmol/g and I〉3. 343 μmol/g were 0. 68（95% CI：O. 50 -0. 93） and 0. 48（95% CI：O. 32 -0. 72 ）compared to those with the level of 〈 0. 825 μmol/g, respectively （both P 〈 0. 05 ）. Conclusion High intake of dietary isothiocyanates could reduce the risk of lung cancer.