HPLC法与微生物法测定人血浆中去甲万古霉素浓度的差异比较

Comparison of Plasma Concentration of Norvancomycin by HPLC and Microbiological Method

目的:比较高效液相色谱(HPLC)法与微生物法测定人血浆中去甲万古霉素浓度的差异。方法:分别使用HPLC法与微生物法对人血浆中去甲万古霉素浓度进行检测,回顾性分析两种技术的临床测结果。结果:两种方法所测得结果的回归方程为y=0.992 7x+0.155 8(r=0.997 6),比较去甲万古霉素血药浓度的检测结果差异无统计学意义(P>0.05)。结论:HPLC法与微生物法均为较有效且可靠的去甲万古霉素血药浓度检测技术。各医院在对去甲万古霉素进行血药浓度检测时,可根据医院自身的条件选择相应的检测方法。

OBJECTIVE：To explore the differences of plasma concentration of norvancomycin by HPLC and microbiological method. METHODS：Microbiological method and HPLC were used to detect the plasma concentration of norvancomycin,and clinical test result of both techniques was retrospectively analyzed. RESULTS：There were no significant differences in the plasma concentration of norvancomycin by microbiological method and HPLC（y=0.992 7x＋0.155 8,r=0.997 6）（P0.05）. CONCLUSIONS：Both microbiological method and HPLC are more effective and reliable for the plasma concentration detection of norvancomycin. The hospitals can choose corresponding method according to their condition when determining plasma concentration of norvancomycin.