黄连上清片中9种成分的HPLC波长切换法测定

Determination of Nine Compounds in Huanglian Shangqing Tablets by HPLC Combined with Wavelength Switching Method

建立了高效液相色谱波长切换法测定黄连上清片中的9种主要成分。采用Dikma Platisil ODS色谱柱,以甲醇∶0.1%磷酸为流动相,梯度洗脱,切换波长检测,检测波长分别为327 nm（绿原酸、黄芩苷）、240 nm（栀子苷）和254 nm（盐酸小檗碱、大黄素、大黄酚、芦荟大黄素、大黄酸和大黄素甲醚）。上述9种成分均达到基线分离,在相应范围内线性关系良好,平均回收率为98.8%~100.7%,RSD为0.4%~1.3%。

An HPLC combined with wavelength switching method was established for the determination of nine main components in Huanglian Shangqing tablets. A Dikma Platisil ODS column was used, with the mobile phase of methanol ： 0.1% phosphoric acid by gradient elution. The detection wavelength was switched for chlorogenic acid and baicalin （at 327 nm）, geniposide （at 240 nm）, berberine hydrochloride, emodin, chrysophanol, aloe-emodin, rhein and physcion （at 254 nm）, respectively. The results showed that all the nine components were well separated, and the linear relationships were good in their corresponding ranges. Their average recoveries were 98.8 % - 100.7%, with RSDs of 0.4%0 - 1.3%.